LOCUS       Exported                4740 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pEMCJH04, complete sequence.
ACCESSION   AY453632
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Hays JP, Eadie K, Verduin CM, Verbrugh H, van Belkum A.
  TITLE     A novel plasmid (pEMCJH03) isolated from moraxella catarrhalis 
            possibly useful as a cloning and expression vector within this 
            species
  JOURNAL   Plasmid 53 (3), 263-268 (2005)
  PUBMED    15848230
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Hays JP, Eadie K, Verduin CM, Verbrugh H, Van Belkum A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2003) Medical Microbiology and Infectious 
            Diseases, Erasmus Mc, Dr. Molewaterplein 40, Rotterdam POSTBUS 2040,
            The Netherlands
REFERENCE   3  (bases 1 to 4740)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Expression vector pEMCJH04"
                     /lab_host="Moraxella catarrhalis"
                     /mol_type="other DNA"
                     /clone="3.9"
                     /note="derived from plasmid pEMCJH03 containing random 
                     insert of transposon EZ::TN"
                     /db_xref="taxon:260048"
     oriT            92..204
                     /label=putative
                     /note="putative"
     CDS             230..1147
                     /codon_start=1
                     /product="putative replicase"
                     /label=putative replicase
                     /protein_id="AAR88169.1"
                     /translation="MQNINFSYDLALIQAQILEKLPQKPYCTDDLGYLVVRPKEIAIQK
                     RYIQHNPPCFTSFLVFDLDTELGAVAWHDADLPLPTWTTQNPDNGHAHIAYALKTPVVT
                     TEQGSNRAIDYLAKIQAGMARKLGADTGYSGLITKNPLHNHWRTTVWNTKPYELGELAD
                     RVELAPLTPKERELGLGRNCTLFDTVRKWAYIAIREHRGGRYDDWYARVLATVQAENMA
                     FLEPLPYSEIKATAKSIAVYCWKNDGHCYNEFIYRQRLKGAIGGKKSKRPPMVDSERTL
                     QPWIELGISRRTYYRDLNDNHSQK"
     rep_origin      1053..1414
                     /label=putative oriV
                     /note="putative oriV"
     gene            1488..1746
                     /gene="putative mobilization gene C"
                     /label=putative mobilization gene C
                     /note="disrupted by transposon insertion"
     mobile_element  1747..2967
                     /mobile_element_type="transposon:EZ::TN"
     misc_feature    1747..1765
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             1884..2699
                     /codon_start=1
                     /product="aminoglycoside 3-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside 3-phosphotransferase
                     /protein_id="AAR88170.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    2949..2967
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     gene            2976..3058
                     /gene="putative mobilization gene C"
                     /label=putative mobilization gene C
                     /note="disrupted by transposon insertion"
     CDS             3281..4543
                     /codon_start=1
                     /product="putative mobilization protein A"
                     /label=putative mobilization protein A
                     /protein_id="AAR88171.1"
                     /translation="MASFERTLMAGLNQDRYNILWVEHTDKDRLELNFLIPKVDLGTGK
                     AMNPYFDKTDRGLVDVWKQVINYDYGLHDPDDPKNRQTLVTVKDLPKSKQEFKQALTAV
                     LEQKILADEIKDHADIIKELENMGLEIARTTPTAISIKDPDGGRNIRLKGEIYEQTFTA
                     NQATERESQRASESYRNELEQRISRVRDELTSRIEAKSAFNATRYKTIPSREQSPNEQA
                     HGIQDPSRGSNGDFVINPDSIRGVQSVLGQENSHTARAIRRYTTGDRQQPPTSQSTNES
                     TGNGTGRQDLHRQQDEQSQNMAKQRQTTNHGATLNVKAIPERVRAIATRARTLLVIARD
                     GKSDAQATDRAITATNSGLRDRKQQANDRKQRTVEIIGVAKNAGAGIDQHHAEQVRLQQ
                     QQARQARQQTKDEPKKLGLDR"