LOCUS       Exported                3892 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pELI3.1, complete sequence.
ACCESSION   EU044918
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Yero CD, Pajon FR, Caballero ME, Cobas AK, Lopez HY, Farinas MM, 
            Gonzales BS, Acosta DA.
  TITLE     Immunization of mice with Neisseria meningitidis serogroup B genomic
            expression libraries elicits functional antibodies and reduces the 
            level of bacteremia in an infant rat infection model
  JOURNAL   Vaccine 23 (7), 932-939 (2005)
  PUBMED    15603895
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Yero D, Pajon R, Caballero E, Cobas K, Lopez Y, Farinas M, Gonzales 
            S, Acosta A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2007) Molecular Biology, Finlay Institute, Ave 27,
            Havana City, Havana 11600, Cuba
REFERENCE   3  (bases 1 to 3892)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Cloning vector pELI3.1"
                     /mol_type="other DNA"
                     /label=mammalian expression vector
                     /note="mammalian expression vector"
                     /db_xref="taxon:459496"
     regulatory      1..659
                     /regulatory_class="enhancer"
                     /note="cytomegalovirus immediate-early enhancer"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      669..750
                     /regulatory_class="promoter"
                     /note="cytomegalovirus immediate-early promoter"
     intron          890..1022
                     /note="beta globin/IgG chimeric intron"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1099..1197
                     /regulatory_class="promoter"
                     /note="lactose promoter; ATG triplets were mutated"
     protein_bind    1177..1193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1207..1216
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1213..1554
                     /codon_start=1
                     /product="beta-galactosidase alpha subunit"
                     /label=beta-galactosidase alpha subunit
                     /note="LacZ alpha subunit"
                     /protein_id="ABS88289.1"
                     /translation="MALITPSLHACRSTLDVRSDQGILTDTRVPSSNSLAVVLQRRDWE
                     NPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRI
                     GRPLPFSEG"
     misc_feature    1242..1313
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1314..1330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1538..1555)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      1564..1785
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late polyadenylation signal"
     polyA_signal    1573..1694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2091..2195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2196..3056
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="ampicillin resistance"
                     /protein_id="ABS88290.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2196..3056
                     /gene="AmpR"
                     /label=AmpR
     rep_origin      3227..3815
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3248..3828
                     /label=ColE1 origin of replication
                     /note="ColE1 origin of replication"