LOCUS       Exported                5738 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pEGFP-Pax9, complete sequence.
ACCESSION   DQ022572
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     The Construction and Expression of Green Fluorescent Protein (GFP)- 
            Paired-box Gene 9 (Pax9) Fused Protein Eukaryotic Cell Expression 
            Plasmid and Subcellular Location of Pax9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2005) School of Stomatology, Wuhan University, 
            Luoyu Road 65#, Wuhan, Hubei 430079, China
REFERENCE   3  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Expression vector pEGFP-Pax9"
                     /mol_type="other DNA"
                     /db_xref="taxon:331529"
     misc_feature    1..589
                     /note="Human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             613..2394
                     /codon_start=1
                     /product="green fluorescent protein-paired-box gene 9 
                     fusion protein"
                     /label=green fluorescent protein-paired-box gene 9 fus
                     /note="GFP-Pax9"
                     /protein_id="AAY63958.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKSGLRSRAQASNSMEPAFGEVNQLGGVFVNGRPLPNAIRLRIV
                     ELAQLGIRPCDISRQLRVSHGCVSKILARYNETGSILPGAIGGSKPRVTTPTVVKHIRT
                     YKQRDPGIFAWEIRDRLLADGVCDKYNVPSVSSISRILRNKIGNLAQQGHYDSYKQHQP
                     APQPALPYNHIYSYPSPITAAAAKVPTPPGVPAIPGSVALPRTWPSSHSVTDILGIRSI
                     TDQGVSDSSPYHSPKVEEWSSLGRNNFPAAAPHAVNGLEKGALEQEAKYGQAPNGLPAV
                     SSFVSASSMAPYPTPAQVSPYMTYSAAPSGYVAGHGWQHAGSTPLSPHNCDIPASLAFK
                     GMQAAREGSHSVTASAL"
     CDS             613..1329
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2526..2647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2557..2607
                     /label=SV40 early mRNA
                     /note="SV40 early mRNA"
     misc_feature    2654..3109
                     /label=f1 single-strand DNA origin
                     /note="f1 single-strand DNA origin"
     rep_origin      complement(2654..3109)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3136..3240
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3242..3599
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3450..3585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3634..4428
                     /codon_start=1
                     /product="kanamycin/neomycin resistance protein"
                     /label=kanamycin/neomycin resistance protein
                     /protein_id="AAY63959.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4660..4707
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"