LOCUS       Exported                6654 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pEGFP-N1-TRAP1, complete sequence.
ACCESSION   LT726828
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6654)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Mammalian expression vector pEGFP-N1-TRAP1"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 6239."
                     /db_xref="taxon:1945021"
     regulatory      1..568
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             641..2578
                     /codon_start=1
                     /note="unnamed protein product; mature hTRAP1"
                     /protein_id="SJL86614.1"
                     /translation="MSTQTAEDKEEPLHSIISSTESVQGSTSKHEFQAETKKLLDIVAR
                     SLYSEKEVFIRELISNASDALEKLRHKLVSDGQALPEMEIHLQTNAEKGTITIQDTGIG
                     MTQEELVSNLGTIARSGSKAFLDALQNQAEASSKIIGQFGVGFYSAFMVADRVEVYSRS
                     AAPGSLGYQWLSDGSGVFEIAEASGVRTGTKIIIHLKSDCKEFSSEARVRDVVTKYSNF
                     VSFPLYLNGRRMNTLQAIWMMDPKDVGEWQHEEFYRYVAQAHDKPRYTLHYKTDAPLNI
                     RSIFYVPDMKPSMFDVSRELGSSVALYSRKVLIQTKATDILPKWLRFIRGVVDSEDIPL
                     NLSRELLQESALIRKLRDVLQQRLIKFFIDQSKKDAEKYAKFFEDYGLFMREGIVTATE
                     QEVKEDIAKLLRYESSALPSGQLTSLSEYASRMRAGTRNIYYLCAPNRHLAEHSPYYEA
                     MKKKDTEVLFCFEQFDELTLLHLREFDKKKLISVETDIVVDHYKEEKFEDRSPAAECLS
                     EKETEELMAWMRNVLGSRVTNVKVTLRLDTHPAMVTVLEMGAARHFLRMQQLAKTQEER
                     AQLLQPTLEINPRHALIKKLNQLRASEPGLAQLLVDQIYENAMIAAGLVDDPRAMVGRL
                     NELLVKALERH"
     regulatory      2594..2603
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2600..3319
                     /codon_start=1
                     /note="unnamed protein product; EGFP"
                     /protein_id="SJL86615.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      complement(3420..3516)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      3420..3516
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    3442..3563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3570..4025)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     regulatory      4026..4121
                     /regulatory_class="promoter"
                     /note="ampicillin promoter"
     promoter        4052..4156
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4122..4156
                     /label=5 UTR of amp, incl. RBS
                     /note="5 UTR of amp, incl. RBS"
     promoter        4158..4515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4177..4458
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      4366..4501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4415..4490
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             4550..5344
                     /codon_start=1
                     /note="unnamed protein product; Tn5 neomycin resistance"
                     /protein_id="SJL86616.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      5535..5604
                     /regulatory_class="terminator"
                     /note="HSV-TK polyA"
     polyA_signal    5576..5623
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5952..6540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      6322..6648
                     /label=pMB1 ori
                     /note="pMB1 ori"