LOCUS       Exported               10166 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEG202 (pLexA), complete sequence.
ACCESSION   U89960
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     Interaction trap/two-hybrid systems to identify interacting proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     pLexA (pEG202) complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 10166)
  AUTHORS   Golemis E, Gyuris J, Brent R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   4  (bases 1 to 10166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3.
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECHs Technical Support 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="unidentified cloning vector"
                     /mol_type="genomic DNA"
                     /label=commercially available as pLex
                     /note="commercially available as pLex"
                     /db_xref="taxon:45196"
     regulatory      67..1511
                     /regulatory_class="promoter"
                     /note="ADH1 promoter"
     promoter        1110..1511
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1538..2230
                     /codon_start=1
                     /product="LexA"
                     /label=LexA
                     /note="DNA-binding domain"
                     /protein_id="AAB68649.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWLEFPGIRRPWRPLESTCSQAN
                     SGRISYDL"
     CDS             1538..2143
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5-CTGTATATATATACAG-3."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     regulatory      2165..2174
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     terminator      2209..2396
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      3128..4470
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    3460..3507
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(6114..6773)
                     /codon_start=1
                     /product="imidazoleglycerolphosphate dehydratase"
                     /label=imidazoleglycerolphosphate dehydratase
                     /protein_id="AAB68650.1"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     gene            6114..6773
                     /gene="HIS3"
                     /label=HIS3
     promoter        complement(6774..6961)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        8026..8130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8131..8991
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=ampR
                     /protein_id="AAB68651.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            8131..8991
                     /gene="ampR"
                     /label=ampR
     rep_origin      9162..9750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9936..10076
                     /label=bom
                     /note="basis of mobility region from pBR322"