LOCUS       Exported                5568 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEF-FS-EGFP-dneo, complete sequence.
ACCESSION   JF313342
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Wilke S, Gossen M, Wirth D, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Cloning vector pEF-FS-EGFP-dneo"
                     /mol_type="other DNA"
                     /db_xref="taxon:1043695"
     regulatory      111..1300
                     /regulatory_class="promoter"
                     /note="derived from Homo sapiens elongation factor 1 alpha"
     promoter        111..1300
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          341..1291
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_recomb     1322..1369
                     /note="3 site; synthetic variant 3 of the yeast 
                     flp-recombinase target (FRT)"
     regulatory      1382..1391
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1388..2134
                     /codon_start=1
                     /product="enhanced green fluorescence protein EGFP"
                     /label=enhanced green fluorescence protein EGFP
                     /protein_id="AEI54555.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKSGRTQIHRI"
     CDS             1388..2104
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2247..2368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2278..2312
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from SV40 virus"
     misc_recomb     2403..2450
                     /label=wild type flp-recombinase target (FRT) site
                     /note="wild type flp-recombinase target (FRT) site"
     protein_bind    2403..2450
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    2488..3237
                     /label=similar to aminoglycoside phosphotransferase
                     /note="similar to aminoglycoside phosphotransferase"
     promoter        complement(3289..3307)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      3333..3557
                     /regulatory_class="polyA_signal_sequence"
                     /note="derived from bovine growth hormone gene (BGH)"
     polyA_signal    3333..3557
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3757..4430
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      complement(3816..4404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4575..5435)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase precursor"
                     /EC_number="3.5.2.6"
                     /label=bla
                     /note="similar to the beta-lactamase precursor encoded by 
                     INSDC accession number AF074376; penicillinase precursor"
                     /protein_id="AEI54554.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4575..5435)
                     /gene="bla"
                     /label=bla
     promoter        complement(5436..5540)
                     /gene="bla"
                     /label=AmpR promoter