LOCUS       Exported                7909 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pEDMS1 DNA, complete sequence.
ACCESSION   AB571797
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Yamashiro D, Minouchi Y, Ashiuchi M.
  TITLE     Moonlighting Role of a Poly-{gamma}-Glutamate Synthetase Component 
            from Bacillus subtilis: Insight into Novel Extrachromosomal DNA 
            Maintenance
  JOURNAL   Appl. Environ. Microbiol. 77 (8), 2796-2798 (2011)
  PUBMED    21357437
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Ashiuchi M, Yamashiro D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2010) Contact:Makoto Ashiuchi Kochi University, 
            Agricultural Science, Graduate School of Integrated Arts and 
            Sciences; Monobe 200, Nankoku, Kochi 783-8502, Japan
REFERENCE   3  (bases 1 to 7909)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Expression vector pEDMS1"
                     /mol_type="other DNA"
                     /note="It can remain in the host cell almost permanently 
                     keeping multicopy ability, even under the condition without
                     the selective pressure such as antibiotics."
                     /db_xref="taxon:864707"
     CDS             37..204
                     /codon_start=1
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /product="extra-chromosomal DNA-maintenance factor EdmS"
                     /label=edmS
                     /protein_id="BAK19406.1"
                     /translation="MKFVKAIWPFVAVAIVFMFMSAFKFNDQLTDQEKQKIDMEMNKIQ
                     QQEEPVNANK"
     gene            37..204
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /label=edmS
     rep_origin      complement(749..1337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1508..2368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2369..2473)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2637..4013)
                     /codon_start=1
                     /gene="Bacillus subtilis tetL"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(4213..5217)
                     /codon_start=1
                     /gene="repB"
                     /product="RepB replication protein"
                     /label=repB
                     /note="from Enterococcus faecalis plasmid pAM-alpha-1"
                     /db_xref="GI:22652809"
                     /protein_id="AAN03827.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      5368..5395
                     /label=Bacillus subtilis replication origin pBC16: oriU
                     /note="Bacillus subtilis replication origin pBC16: oriU"
     promoter        5642..5670
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"