LOCUS       Exported                4346 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Auxiliary vector pECnpt, complete sequence.
ACCESSION   KY930502
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 4346)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Auxiliary vector pECnpt"
                     /mol_type="other DNA"
                     /db_xref="taxon:2127036"
     regulatory      6..227
                     /regulatory_class="terminator"
                     /note="psbA terminator; TpsbA"
     primer_bind     complement(258..274)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    282..298
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(306..336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    351..372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(660..1248)
                     /direction=LEFT
                     /label=pUC19 replication origin
                     /note="pUC19 replication origin"
     CDS             complement(1419..2279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers ampicillin resistance"
                     /protein_id="AVQ67773.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1419..2279)
                     /gene="bla"
                     /label=bla
     promoter        complement(2280..2384)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2858..2874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2887..3134
                     /regulatory_class="promoter"
                     /note="atpI promoter; PatpI"
     misc_feature    3135..3150
                     /label=cloning site
                     /note="cloning site"
     regulatory      3151..3319
                     /regulatory_class="terminator"
                     /note="rps16 terminator; Trps16"
     regulatory      3326..3544
                     /regulatory_class="promoter"
                     /note="rrn promoter; Prrn"
     CDS             3551..4345
                     /codon_start=1
                     /gene="nptII"
                     /product="neomycin phosphotransferase II"
                     /label=nptII
                     /note="confers kanamycin resistance"
                     /protein_id="AVQ67774.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3551..4345
                     /gene="nptII"
                     /label=nptII