LOCUS       Exported                5513 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pECFP-YFP, complete sequence.
ACCESSION   LT726795
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5513)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Mammalian expression vector pECFP-YFP"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 8242."
                     /db_xref="taxon:1944966"
     regulatory      1..568
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..616
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             613..1329
                     /codon_start=1
                     /note="unnamed protein product; ECFP"
                     /protein_id="SJL86504.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1369..1392
                     /label=Xpress epitope
                     /note="Xpress epitope"
     CDS             1369..1392
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     misc_feature    1378..1392
                     /label=EK recognition site
                     /note="EK recognition site"
     CDS             1411..2124
                     /codon_start=1
                     /note="unnamed protein product; YFP"
                     /protein_id="SJL86505.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      complement(2279..2375)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2279..2375
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    2301..2422
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2429..2884)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     regulatory      2885..2980
                     /regulatory_class="promoter"
                     /note="ampicillin resistance promoter"
     promoter        2911..3015
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2981..3015
                     /label=5 UTR of amp, incl. RBS
                     /note="5 UTR of amp, incl. RBS"
     promoter        3017..3374
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      3036..3317
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      3225..3360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      3274..3349
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             3409..4203
                     /codon_start=1
                     /note="unnamed protein product; Tn5 neomycin resistance"
                     /protein_id="SJL86506.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      4394..4463
                     /regulatory_class="terminator"
                     /note="HSV-TK polyA"
     polyA_signal    4435..4482
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4811..5399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5181..5507
                     /label=pMB1 ori
                     /note="pMB1 ori"