LOCUS       Exported                4343 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Auxiliary vector pECaad, complete sequence.
ACCESSION   KY930501
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 4343)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Auxiliary vector pECaad"
                     /mol_type="other DNA"
                     /db_xref="taxon:2127035"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      408..655
                     /regulatory_class="promoter"
                     /note="atpI promoter; PatpI"
     misc_feature    656..671
                     /label=cloning site
                     /note="cloning site"
     regulatory      672..840
                     /regulatory_class="terminator"
                     /note="rps16 terminator; Trps16"
     regulatory      847..1065
                     /regulatory_class="promoter"
                     /note="rrn promoter; Prrn"
     CDS             1072..1863
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside 3-adenyltransferase"
                     /label=aadA
                     /note="confers spectinomycin resistance"
                     /protein_id="AVQ67771.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1072..1863
                     /gene="aadA"
                     /label=aadA
     regulatory      1870..2091
                     /regulatory_class="terminator"
                     /note="psbA terminator; TpsbA"
     primer_bind     complement(2122..2138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2146..2162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2170..2200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2215..2236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=pUC19 replication origin
                     /note="pUC19 replication origin"
     CDS             complement(3283..4143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="confers ampicillin resistance"
                     /protein_id="AVQ67772.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3283..4143)
                     /gene="bla"
                     /label=bla
     promoter        complement(4144..4248)
                     /gene="bla"
                     /label=AmpR promoter