LOCUS       Exported               14553 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEAVrVBS, complete sequence.
ACCESSION   DQ846751
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Balasuriya UB, Snijder EJ, Heidner HW, Zhang J, Zevenhoven-Dobbe JC,
            Boone JD, McCollum WH, Timoney PJ, Maclachlan NJ.
  TITLE     Development and characterization of an infectious cDNA clone of the 
            virulent Bucyrus strain of Equine arteritis virus
  JOURNAL   J. Gen. Virol. 88 (PT 3), 918-924 (2007)
  PUBMED    17325365
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Balasuriya UB, MacLachlan J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2006) Veterinary Science, University of Kentucky, 
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14553)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Cloning vector pEAVrVBS"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:400350"
     source          1..12704
                     /organism="Equine arteritis virus"
                     /strain="Bucyrus; ATCC VR-796"
                     /mol_type="other DNA"
                     /label=virulent strain EAVVBS
                     /note="virulent strain EAVVBS"
                     /db_xref="taxon:11047"
     CDS             225..5399
                     /codon_start=1
                     /product="replicase polyprotein"
                     /label=replicase polyprotein
                     /note="ORF1a"
                     /protein_id="ABI64080.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYASAWVASPAANSLIVTIDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFDNVRVVTPEGQPRPVPAP
                     RVRPSANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPLKTPAVQRYTMTLKMMRS
                     RFSWHCDVWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNSVVLTALLVSSANYVASM
                     DHQCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCRNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VTKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTMLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSNDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQ"
     misc_feature    5399..5405
                     /note="site of ribosomal frameshift; slippery sequence"
     CDS             5405..9751
                     /codon_start=1
                     /product="replicase polyprotein"
                     /label=replicase polyprotein
                     /note="ORF1b; translated from ORF1a by programmed -1 
                     ribosomal frameshift"
                     /protein_id="ABI64088.1"
                     /translation="LRAPHIFPGDVGRRTFADSKDKGFVALHSRTMFLAARDFLFNIKF
                     VCDEEFTKTPKDTLLGYVRACPGYWFIFRRTHRSLIDAYWDSMECVYALPTISDFDVSP
                     GDVAVTGERWDFESPGGGRAKRLTADLVHAFQGFHGASYSYDDKVAAAVSGDPYRSDGV
                     LYNTRWGNIPYSVPTNALEATACYRAGCEAVTDGTNVIATIGPFPEQQPIPDIPKSVLD
                     NCADISCDAFIAPAAETALCGDLEKYNLSTQGFVLPSVFSMVRAYLKEEIGDAPPLYLP
                     STVPSKNSQAGINGAEFPTKSLQSYCLIDDMVSQSMKSNLQTATMATCKRQYCSKYKIR
                     SILGTNNYIGLGLRACLSGVTAAFQKAGKDGSPIYLGKSKFDPIPAPDKYCLETDLESC
                     DRSTPALVRWFATNLIFELAGQPELVHSYVLNCCHDLVVAGSVAFTKRGGLSSGDPITS
                     ISNTIYSLVLYTQHMLLCGLEGYFPEIAEKYLDGSLELRDMFKYVRVYIYSDDVVLTTP
                     NQHYAASFDRWVPHLQALLGFKVDPKKTVNTSSPSFLGCRFKQVDGKCYLASLQDRVTR
                     SLLYHIGAKNPSEYYEAAVSIFKDSIICCDEDWWTDLHRRISGAARTDGVEFPTIEMLT
                     SFRTKQYESAVCTVCGAAPVAKSACGGWFCGNCVPYHAGHCHTTSLFANCGHDIMYRST
                     YCTMCEGSPKQMVPKVPHPILDHLLCHIDYGSKEELTLVVADGRTTSPPGRYKVGHKVV
                     AVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAASEYVEGPPGSGKTFHLV
                     KDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTVLDFPRPGSGNITVRLP
                     QVGTSEGETFVDEVAYFSPVDLARILTQGRVKGYGDLNQLGCVGPASVPRNLWLRHFVC
                     LEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFEKGVVITAYHKDRGLGH
                     RTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIYDPFDQLSGLLKFTKEA
                     EAQDLIHGPPTACHLGQEIDLWSNEGLEYYKEVNLLYTHVPIKDGVIHSYPNCGPACGW
                     EKQSNKISCLPRVAQNLGYHYSPDLPGFCPIPKELAEHWPVVSNDRYPNCLQITLQQVC
                     ELSKPCSAGYMVGQSVFVQTPGVTSYWLTEWVDGKARALPDSLFSSGRFETNSRAFLDE
                     AEEKFAAAHPHACLGEINKSTVGGSHFIFSQYLPPLLPADAVALVGASLAGKAAKAACS
                     VVDVYAPSFEPYLHPETLSRVYKIMIDFKPCRLMVWRNATFYVQEGVDAVTSALAAVSK
                     LIKVPANEPVSFHVASGYRTNALVAPQAKISIGAYAAEWALSTEPPPAGYAIVRRYIVK
                     RLLSSTEVFLCRRGVVSSTSVQTICALEGCKPLFNFLQIGSVIGPV"
     regulatory      6374..6383
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9751..9954
                     /codon_start=1
                     /product="envelope protein E"
                     /label=envelope protein E
                     /note="ORF2a"
                     /protein_id="ABI64083.1"
                     /translation="MGLVWSLISNSIQTIIADFAISVIDAALFFLMLLALAVVTVFLFW
                     LIVAIGRSLVARCSRGARYRPV"
     CDS             9824..10507
                     /codon_start=1
                     /product="glycoprotein 2b"
                     /label=glycoprotein 2b
                     /note="ORF2b; GP2b"
                     /protein_id="ABI64081.1"
                     /translation="MQRFSFSCYLHWLLLLCFFSGSLLPSAAAWWRGVHEVRVTDLFKD
                     LQCDNLRAKDAFPSLGYALSIGQSRLSYMLQDWLLAAHRKEVMPSNIMPMPGLTPDCFD
                     HLESSSYAPFINAYRQAILSQYSQELLLEAINCKLLAVVAPALYHNYHLANLTGPATWV
                     VPTVGQLHYYASSSIFASSVEVLAAIILLFACIPLVTRVYISFTRLMSPSRRTSSGTLP
                     RRKIL"
     CDS             10306..10797
                     /codon_start=1
                     /product="glycoprotein 3"
                     /label=glycoprotein 3
                     /note="ORF3; GP3"
                     /protein_id="ABI64084.1"
                     /translation="MGRAYSGPVALLCFFLYFCFICGSVGSNNTTICMHTTSDTSVHLF
                     YAANVTFPSHFQRHFAAAQDFVVHTGYEYAGVTMLVHLFANLVLTFPSLVNCSRPVNVF
                     ANASCVQVVCSHTNSTTGLGQLSFSFVDEDLRLHIRPTLICWFALLLVHFLPMPRCRGS
                     "
     CDS             10700..11158
                     /codon_start=1
                     /product="glycoprotein 4"
                     /label=glycoprotein 4
                     /note="ORF4; GP4"
                     /protein_id="ABI64085.1"
                     /translation="MKIYGCILGLLLFVGLPCCWCTFYPCHAAEARNFTYISHGLGHVH
                     GHEGCRNFINVTHSAFLYLNPTTLTAPAITHCLLLVLAAKMEHPNATIWLQLQPFGYHV
                     AGDVIVNLEENKRHPYFKLLRAPALPLGFVAIVYVLLRLVRWAQQCYL"
     CDS             11146..11913
                     /codon_start=1
                     /product="glycoprotein 5"
                     /label=glycoprotein 5
                     /note="ORF5; GP5"
                     /protein_id="ABI64082.1"
                     /translation="MLSMIVLLFLLWGAPSHAYFSYYTAQRFTDFTLCMLTDRGVIANL
                     LRYDEHTALYNCSASKTCWYCTFLDEQIITFGTDCNDTYAVPVAEVLEQAHGPYSVLFN
                     DMPPFIYYGREFGIVVLDVFMFYPVLVLFFLSVLPYATLILEMCVSILFIIYGIYSGAY
                     LAMGIFAATLAIHSIVVLRQLLWLCLAWRYRCTLHASFISAEGKVYPVDPGLPVAAAGN
                     RLLVPGRPTIDYAVAYGSKVNLVRLGAAEVWEP"
     CDS             11901..12389
                     /codon_start=1
                     /product="unglycosylated membrane protein M"
                     /label=unglycosylated membrane protein M
                     /note="ORF6"
                     /protein_id="ABI64086.1"
                     /translation="MGAIDSFCGDGILGEYLDYFILSVPLLLLLTRYVASGLVYVLTAL
                     FYSFVLAAYIWFVIVGRAFSTAYAFVLLAAFLLLVMRMIVGMMPRLRSIFNHRQLVVAD
                     FVDTPSGPVPIPRSTTQIVVRGNGYTAVGNKLVDGVKTITSAGRLFSKRAAATAYKLQ"
     CDS             12313..12645
                     /codon_start=1
                     /product="nucleocapsid protein N"
                     /label=nucleocapsid protein N
                     /note="ORF7"
                     /protein_id="ABI64087.1"
                     /translation="MASRRSRPQAASFRNGRRRQPTSYNDLLRMFGQMRVRKPPAQPTQ
                     AIIAEPGDLRHDLNQQERATLSSNVQRFFMIGHGSLTADAGGLTYTVSWVPTKQIQRKV
                     APPAGP"
     promoter        12785..12889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12890..13750
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERNTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13921..14509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        14537..1
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"