LOCUS       Exported               14622 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEAVrMLVB-1b2a-MCS, complete sequence.
ACCESSION   HM446468
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Zhang J, Go YY, Lu Z, Meade BJ, Timoney PJ, Balasuriya UBR.
  TITLE     Development and Characterization of an Infectious cDNA Clone of the 
            Modified Live Virus Vaccine Strain of Equine Arteritis Virus and Its
            Potential As a Vaccine Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2010) Veterinary Science, University of Kentucky, 
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14622)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Cloning vector pEAVrMLVB-1b2a-MCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1037914"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage
                     /product="replicase polyprotein 1ab"
                     /label=replicase polyprotein 1ab
                     /note="ORF1ab"
                     /protein_id="AEG80297.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLNLRAPHIFPGDVGRRTFADSKDKGFVALHS
                     RTMFLAARDFLFNIKFVCDEEFTKTPKDTLLGYVRACPGYWFIFRRTHRSLIDAYWDSM
                     ECVYALPTISDFDVSPGDVAVTGERWDFESPGGGRAKRLTADLVHAFQGFHGASYSYDD
                     KVAAAVSGDPYRSDGVLYNTRWGNIPYSVPTNALEATACYRAGCEAVTDGTNVIATIGP
                     FPEQQPIPDIPKSVLDNCADISCDAFIAPAAETALCGDLEKYNLSTQGFVLPSVFSMVR
                     AYLKEEIGDAPPLYLPSTVPSKNSQAGINGAEFPTRSLQSYCLIDDMVSQSMKSNLQTA
                     TMATCKRQYCSKYKIRSILGTNNYIGLGLRACLSGVTAAFQKAGKDGSPIYLGKSKFDP
                     IPAPDKYCLETDLESCDRSTPALVRWFATNLIFELAGQPELVHSYVLNCCHDLVVAGSV
                     AFTKRGGLSSGDPITSISNTIYSLVLYTQHMLLCGLEGYFPEIAEKYLDGSLELRDMFK
                     YVRVYIYSDDVVLTTPNQHYAASFDRWVPHLQALLGFKVDPKKTVNTSSPSFLGCRFKQ
                     VDGKCYLASLQDRVTRSLLYHIGAKNPSEYYEAAVSIFKDSIICCDEDWWTDLHRRISG
                     AARTDGVEFPTIEMLTSFRTKQYESAVCTVCGAAPVAKSACGGWFCGNCVPYHVGHCHT
                     TSLFANCGHDIMYRSTYCTMCEGSPKQMVPKVPHPILDHLLCHVDYGSKEELTLVVADG
                     RTTSPPGRYKVGHKVVAVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAAS
                     EYVEGPPGSGKTFHLVKDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTV
                     LDFPRPGSGNITVRLPQVGTSEGETFVDEVAYFSPVDLARILTQGRVKGYGDLNQLGCV
                     GPASVPRNLWLRHFVSLEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFE
                     KGVVITAYHKDRGLGHRTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIY
                     DPFDQLSGLLKFTKEAEAQDLIHGPPTACHLGQEIDLWSNEGLEYYKEVNLLYTHVPIK
                     DGVIHSYPNCGPACGWEKQSNKISCLPRVAQNLGYHYSPDLPGFCPIPKELAEHWPVVS
                     NDRYPNCLQITLQQVCELSKPCSAGYMVGQSVFVQTPGVTSYWLTEWVDGKARALPDSL
                     FSSGRFETNSRVFLDEAEEKFAAAHPHACLGEINKSTVGGSHFIFSQYLPPLLPAGAVA
                     LVGASLAGKAAKAACSVVDVYAPSFEPYLHPETLSRVYKIMIDFKPCRLMVWRNATFYV
                     QEGVDAVTSALAAVSKLIKVPANEPVSFHVASGYRTNALVAPQAKISIGAYAAEWALST
                     EPPPAGYAIVRRYIVKRLLSSTEVFLCRRGVVSSTSVQTICALEGCKPLFNFLQIGSVI
                     GPV"
     CDS             225..5408
                     /codon_start=1
                     /product="replicase polyprotein 1a"
                     /label=replicase polyprotein 1a
                     /note="ORF1a"
                     /protein_id="AEG80298.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     regulatory      6374..6383
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9820..10023
                     /codon_start=1
                     /product="envelope protein E"
                     /label=envelope protein E
                     /note="ORF2a"
                     /protein_id="AEG80299.1"
                     /translation="MGLVWSLISNSIQTIIADFAISVIDAALFFLMLLALAVVTVFLFW
                     LIVAIGRSLVARCSRGARYRPV"
     CDS             9893..10576
                     /codon_start=1
                     /product="glycoprotein 2 GP2"
                     /label=glycoprotein 2 GP2
                     /note="ORF2b"
                     /protein_id="AEG80300.1"
                     /translation="MQRFSFSCYLHWLLLLCFFSGSLLPSAAAWWRGVHEVRVTDLFKD
                     LQCDNLRAKDAFPSLGHALSIGQSRLSYMLQDWLLAAHRKEVMPSNTMPMPGLTPDCFD
                     HLESSSYAPFINAYRQAILSQYSQELLLEAINCKLLAVVAPALYHNYHLANLTEPATWV
                     VPTVGQLHYYASSSIFASSVEVLAAIILLFACIPLVTRVYISFTRLMSPSRRTSSGTLP
                     PRKIL"
     CDS             10375..10881
                     /codon_start=1
                     /product="glycoprotein 3 GP3"
                     /label=glycoprotein 3 GP3
                     /note="ORF3"
                     /protein_id="AEG80301.1"
                     /translation="MGRAYSGPVALLCFFLYFCFICGSVGSNNTTICMHTTSDTSVHLF
                     YAANVTFPSHFQRHFAAAQDFVVHTGYEYAGVTMVVHLFANLVLTFPSLVNCSRPVNVF
                     ANASCVQVVCSHTNSTTGSGQLSFSFVDEDLRLHIRPTLICWFALLLVHFLPMPRYRGS
                     QFYLH"
     CDS             10769..11227
                     /codon_start=1
                     /product="glycoprotein 4 GP4"
                     /label=glycoprotein 4 GP4
                     /note="ORF4"
                     /protein_id="AEG80302.1"
                     /translation="MKISGCISGLLLFVGLPCCWCTFYPCHATEARNFTYTSHGLGHVH
                     GHEGCRNFINVTHSAFLYLNPTTLTAPAITHCLLLVLAAKMEHPNATIWLQLQPFGYHV
                     AGDVIVNLEENKRHPYFKLLRAPALPLGFVAIVYVLLRLVRWAQQCYL"
     CDS             11215..11982
                     /codon_start=1
                     /product="glycoprotein 5 GP5"
                     /label=glycoprotein 5 GP5
                     /note="ORF5"
                     /protein_id="AEG80303.1"
                     /translation="MLSMIVLLFLLWGAPSHAYFSYYTAQRFTDFTLCMLTDRGVIANL
                     LRYDEHTALYNCSASKTCWYCTFPDEKIITFGTDCDDTYAVPVAEVLEQAHGPYGVLFG
                     DVPPFIYYGREFGIVVLDVFMFYPVLVLFFLSVLPYATLILEMCVSILFIIYGIYSGAY
                     LAMGIFSATLAIHSIVVLRQLLWLCLAWRYRCTLHASFISAEGKVYPVDPELPVAAAGN
                     RLLVPGRPTIDYAVAYGSKVNLVRLGAAEVWEP"
     CDS             11970..12458
                     /codon_start=1
                     /product="unglycosylated membrane protein M"
                     /label=unglycosylated membrane protein M
                     /note="ORF6"
                     /protein_id="AEG80304.1"
                     /translation="MGAIDSFCGDGILGEYLDYFILSVPLLLLLTRYVASGSVYVLTAL
                     FYSLVLAAYIWFVIVGRAFSTAYAFALLAAFLLLVTRMIVGMMPRLRSIFNHRQLVVAD
                     FVDTPSGPVPIPRSTTQVVVRGNGYTAVGNKLVDGVKTITSAGRLCSKRMAATAYKLQ"
     CDS             12382..12714
                     /codon_start=1
                     /product="nucleocapsid protein N"
                     /label=nucleocapsid protein N
                     /note="ORF7"
                     /protein_id="AEG80305.1"
                     /translation="MASRRSRPQAASVRNGWRRQPTSYNDLLRMFGQMRVRKPPAQPTQ
                     AIIAEPGDLRHDLNQQERATLSSNVQRFFMIGHGSLTADAGGLTYTVSWVPTKQIQRKI
                     APPAGP"
     promoter        12854..12958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12959..13819
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13990..14578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        14606..1
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"