LOCUS       Exported               14553 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEAVrMLV, complete sequence.
ACCESSION   FJ798195
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Zhang J, Go YY, Huang CM, Meade BJ, Lu Z, Snijder EJ, Timoney PJ, 
            Balasuriya UB.
  TITLE     Development and characterization of an infectious cDNA clone of the 
            modified live virus vaccine strain of equine arteritis virus
  JOURNAL   Clin. Vaccine Immunol. 19 (8), 1312-1321 (2012)
  PUBMED    22739697
REFERENCE   2  (bases 1 to 14553)
  AUTHORS   Zhang J, Balasuriya UBR.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2009) Veterinary Science, University of Kentucky, 
            108 Gluck Equine Research Center, Lexington, KY 40546, USA
REFERENCE   3  (bases 1 to 14553)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Cloning vector pEAVrMLV"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:690568"
     source          1..12704
                     /organism="Equine arteritis virus"
                     /mol_type="other DNA"
                     /db_xref="taxon:11047"
     CDS             225..9751
                     /codon_start=1
                     /ribosomal_slippage
                     /gene="ORF1ab"
                     /product="replicase polyprotein 1ab"
                     /label=ORF1ab
                     /protein_id="ACY79505.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLNLRAPHIFPGDVGRRTFADSKDKGFVALHS
                     RTMFLAARDFLFNIKFVCDEEFTKTPKDTLLGYVRACPGYWFIFRRTHRSLIDAYWDSM
                     ECVYALPTISDFDVSPGDVAVTGERWDFESPGGGRAKRLTADLVHAFQGFHGASYSYDD
                     KVAAAVSGDPYRSDGVLYNTRWGNIPYSVPTNALEATACYRAGCEAVTDGTNVIATIGP
                     FPEQQPIPDIPKSVLDNCADISCDAFIAPAAETALCGDLEKYNLSTQGFVLPSVFSMVR
                     AYLKEEIGDAPPLYLPSTVPSKNSQAGINGAEFPTRSLQSYCLIDDMVSQSMKSNLQTA
                     TMATCKRQYCSKYKIRSILGTNNYIGLGLRACLSGVTAAFQKAGKDGSPIYLGKSKFDP
                     IPAPDKYCLETDLESCDRSTPALVRWFATNLIFELAGQPELVHSYVLNCCHDLVVAGSV
                     AFTKRGGLSSGDPITSISNTIYSLVLYTQHMLLCGLEGYFPEIAEKYLDGSLELRDMFK
                     YVRVYIYSDDVVLTTPNQHYAASFDRWVPHLQALLGFKVDPKKTVNTSSPSFLGCRFKQ
                     VDGKCYLASLQDRVTRSLLYHIGAKNPSEYYEAAVSIFKDSIICCDEDWWTDLHRRISG
                     AARTDGVEFPTIEMLTSFRTKQYESAVCTVCGAAPVAKSACGGWFCGNCVPYHVGHCHT
                     TSLFANCGHDIMYRSTYCTMCEGSPKQMVPKVPHPILDHLLCHVDYGSKEELTLVVADG
                     RTTSPPGRYKVGHKVVAVVADVGGNIVFGCGPGSHIAVPLQDTLKGVVVNKALKNAAAS
                     EYVEGPPGSGKTFHLVKDVLAVVGSATLVVPTHASMLDCINKLKQAGADPYFVVPKYTV
                     LDFPRPGSGNITVRLPQVGTSEGETFVDEVAYFSPVDLARILTQGRVKGYGDLNQLGCV
                     GPASVPRNLWLRHFVSLEPLRVCHRFGAAVCDLIKGIYPYYEPAPHTTKVVFVPNPDFE
                     KGVVITAYHKDRGLGHRTIDSIQGCTFPVVTLRLPTPQSLTRPRAVVAVTRASQELYIY
                     DPFDQLSGLLKFTKEAEAQDLIHGPPTACHLGQEIDLWSNEGLEYYKEVNLLYTHVPIK
                     DGVIHSYPNCGPACGWEKQSNKISCLPRVAQNLGYHYSPDLPGFCPIPKELAEHWPVVS
                     NDRYPNCLQITLQQVCELSKPCSAGYMVGQSVFVQTPGVTSYWLTEWVDGKARALPDSL
                     FSSGRFETNSRVFLDEAEEKFAAAHPHACLGEINKSTVGGSHFIFSQYLPPLLPAGAVA
                     LVGASLAGKAAKAACSVVDVYAPSFEPYLHPETLSRVYKIMIDFKPCRLMVWRNATFYV
                     QEGVDAVTSALAAVSKLIKVPANEPVSFHVASGYRTNALVAPQAKISIGAYAAEWALST
                     EPPPAGYAIVRRYIVKRLLSSTEVFLCRRGVVSSTSVQTICALEGCKPLFNFLQIGSVI
                     GPV"
     gene            225..9751
                     /gene="ORF1ab"
                     /label=ORF1ab
     CDS             225..5408
                     /codon_start=1
                     /gene="ORF1a"
                     /product="replicase polyprotein 1a"
                     /label=ORF1a
                     /protein_id="ACY79506.1"
                     /translation="MATFSATGFGGSFVRDWSLDLPDACEHGAGLCCEVDGSTLCAECF
                     RGCEGVEQCPGLFMGLLKLASPVPVGHKFLIGWYRAAKVTGRYNFLELLQHPAFAQLRV
                     VDARLAIEEASVFISTDHASAKRFPGARFALTPVYANAWVVSPAANSLIVTTDQEQDGF
                     CWLKLLPPDRREAGLRLYYNHYREQRTGWLSKTGLRLWLGDLGLGINASSGGLKFHIMR
                     GSPQRAWHITTRSCKLKSYYVCDISEADWSCLPAGNYGGYNPPGDGACGYRCLAFMNGA
                     TVVSAGCSSDLWCDDELAYRVFQLSPTFTVTIPGGRVCPNAKYAMICDKQHWRVKRAKG
                     VGLCLDESCFRGTCNCQRMSGPPPAPVSAAVLDHILEAATFGNVRVVIPEGQPRPVPAP
                     RVHPCANSSGDVKDPAPVPPVPKPRTKLAKPNPTQAPIPAPRTRLQGASTQEPLASAGV
                     ASDSAPKWRVAKTVYSSAERFRTELVQRARSVGDVLVQALPHKTPAVQRYTMTLKMMRS
                     RFSWHCDMWYPLAVIACLLPIWPSLALLLSFAIGLIPSVGNNVVLTALLVSSANYVASM
                     DHHCEGAACLALLEEEHYYRAVRWRPITGALSLVLNLLGQVGYVARSTFDAAYVPCTVF
                     DLCSFAILYLCCNRCWRCFGRCVRVGPATHVLGSTGQRVSKLALIDLCDHFSKPTIDVV
                     GMATGWSGCYTGTAAMERQCASTVDPHSFDQKKAGAIVYLTPPVNSGSALQCLNVMWKR
                     PIGSTVLGEQTGAVVTAVKSISFSPPCCVSTTLPTRPGVTVVDHALYNRLTASGVDPAL
                     LRVGQGDFLKLNPGFRLIGGWIYGICYFVLVVVSTFTCLPIKCGIGTRDPFCRRVFSVP
                     VIKTQEHCHAGMCASAEGISLDSLGLTQLQSYWIAAVTSGLVILLVCHRLAISALDLLT
                     LASPLVLLVFPWASVGLLLACSLAGAAVKIQLLATLFVNLFFPQATLVTMGYWACVAAL
                     AVYSLMGLRVKVNVPMCVTPAHFLLLARSAGQSREQMLRVSAAAPTNSLLGVARDCYVT
                     GTTRLYIPKEGGMVFEGLFRSPKARGNVGFVAGSSYGTGSVWTRNNEVVVLTASHVVGR
                     ANMATLKIGDAMLTLTFKKNGDFAEAVTTQSELPGNWPQLHFAQPTTGPASWCTATGDE
                     EGLLSGEVCLAWTTSGDSGSAVVQGDAVVGVHTGSNTSGVAYVTTPSGKLLGADTVTLS
                     SLSKHFTGPLTSIPKDIPDNIIADVDAVPRSLAMLIDGLSNRESSLSGPQLLLIACFMW
                     SYLNQPAYLPYVLGFFAANFFLPKSVGRPVVTGLLWLCCLFTPLSMRLCLFHLVCATVT
                     GNVISLWFYITAAGTSYLSEMWFGGYPTLLFVPRFLVYQFPGWAIGTVLAVCSITMLAA
                     ALGHTLLLDVFSASGRFDRTFMMKYFLEGGVKESVTASVTRAYGKPITQESLTATLAAL
                     TDDDFQFLSDVLDCRAVRSAMNLRAALTSFQVAQYRNILNASLQVDRDAARSRRLMAKL
                     ADFAVEQEVTAGDRVVVIDGLDRMAHFKDDLVLVPLTTKVVGGSRCTICDVVKEEANDT
                     PVKPMPSRRRRKGLPKGAQLEWDRHQEEKRNAGDDDFAVSTDYVKRVPKYWDPSDTRGT
                     TVKIAGTTYQKVVDYSGNVHYVEHQEDLLDYVLGKGSYEGLDQDKVLDLTNMLKVDPTE
                     LSSKDKAKARQLAHLLLDLANPVEAVNQLN"
     gene            225..5408
                     /gene="ORF1a"
                     /label=ORF1a
     regulatory      6374..6383
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9751..9954
                     /codon_start=1
                     /gene="ORF2a"
                     /product="envelope protein"
                     /label=ORF2a
                     /note="E"
                     /protein_id="ACY79507.1"
                     /translation="MGLVWSLISNSIQTIIADFAISVIDAALFFLMLLALAVVTVFLFW
                     LIVAIGRSLVARCSRGARYRPV"
     gene            9751..9954
                     /gene="ORF2a"
                     /label=ORF2a
     CDS             9824..10507
                     /codon_start=1
                     /gene="ORF2b"
                     /product="glycoprotein 2"
                     /label=ORF2b
                     /note="GP2"
                     /protein_id="ACY79508.1"
                     /translation="MQRFSFSCYLHWLLLLCFFSGSLLPSAAAWWRGVHEVRVTDLFKD
                     LQCDNLRAKDAFPSLGHALSIGQSRLSYMLQDWLLAAHRKEVMPSNTMPMPGLTPDCFD
                     HLESSSYAPFINAYRQAILSQYSQELLLEAINCKLLAVVAPALYHNYHLANLTEPATWV
                     VPTVGQLHYYASSSIFASSVEVLAAIILLFACIPLVTRVYISFTRLMSPSRRTSSGTLP
                     PRKIL"
     gene            9824..10507
                     /gene="ORF2b"
                     /label=ORF2b
     CDS             10306..10812
                     /codon_start=1
                     /gene="ORF3"
                     /product="glycoprotein 3"
                     /label=ORF3
                     /note="GP3"
                     /protein_id="ACY79509.1"
                     /translation="MGRAYSGPVALLCFFLYFCFICGSVGSNNTTICMHTTSDTSVHLF
                     YAANVTFPSHFQRHFAAAQDFVVHTGYEYAGVTMVVHLFANLVLTFPSLVNCSRPVNVF
                     ANASCVQVVCSHTNSTTGSGQLSFSFVDEDLRLHIRPTLICWFALLLVHFLPMPRYRGS
                     QFYLH"
     gene            10306..10812
                     /gene="ORF3"
                     /label=ORF3
     CDS             10700..11158
                     /codon_start=1
                     /gene="ORF4"
                     /product="glycoprotein 4"
                     /label=ORF4
                     /note="GP4"
                     /protein_id="ACY79510.1"
                     /translation="MKISGCISGLLLFVGLPCCWCTFYPCHATEARNFTYTSHGLGHVH
                     GHEGCRNFINVTHSAFLYLNPTTLTAPAITHCLLLVLAAKMEHPNATIWLQLQPFGYHV
                     AGDVIVNLEENKRHPYFKLLRAPALPLGFVAIVYVLLRLVRWAQQCYL"
     gene            10700..11158
                     /gene="ORF4"
                     /label=ORF4
     CDS             11146..11913
                     /codon_start=1
                     /gene="ORF5"
                     /product="glycoprotein 5"
                     /label=ORF5
                     /note="GP5"
                     /protein_id="ACY79511.1"
                     /translation="MLSMIVLLFLLWGAPSHAYFSYYTAQRFTDFTLCMLTDRGVIANL
                     LRYDEHTALYNCSASKTCWYCTFPDEKIITFGTDCDDTYAVPVAEVLEQAHGPYGVLFG
                     DVPPFIYYGREFGIVVLDVFMFYPVLVLFFLSVLPYATLILEMCVSILFIIYGIYSGAY
                     LAMGIFSATLAIHSIVVLRQLLWLCLAWRYRCTLHASFISAEGKVYPVDPELPVAAAGN
                     RLLVPGRPTIDYAVAYGSKVNLVRLGAAEVWEP"
     gene            11146..11913
                     /gene="ORF5"
                     /label=ORF5
     CDS             11901..12389
                     /codon_start=1
                     /gene="ORF6"
                     /product="unglycosylated membrane protein"
                     /label=ORF6
                     /note="M"
                     /protein_id="ACY79512.1"
                     /translation="MGAIDSFCGDGILGEYLDYFILSVPLLLLLTRYVASGSVYVLTAL
                     FYSLVLAAYIWFVIVGRAFSTAYAFALLAAFLLLVTRMIVGMMPRLRSIFNHRQLVVAD
                     FVDTPSGPVPIPRSTTQVVVRGNGYTAVGNKLVDGVKTITSAGRLCSKRMAATAYKLQ"
     gene            11901..12389
                     /gene="ORF6"
                     /label=ORF6
     CDS             12313..12645
                     /codon_start=1
                     /gene="ORF7"
                     /product="nucleocapsid protein"
                     /label=ORF7
                     /note="N"
                     /protein_id="ACY79513.1"
                     /translation="MASRRSRPQAASVRNGWRRQPTSYNDLLRMFGQMRVRKPPAQPTQ
                     AIIAEPGDLRHDLNQQERATLSSNVQRFFMIGHGSLTADAGGLTYTVSWVPTKQIQRKI
                     APPAGP"
     gene            12313..12645
                     /gene="ORF7"
                     /label=ORF7
     promoter        12785..12889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             12890..13750
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13921..14509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        14537..1
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"