LOCUS       Exported                3030 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pE5n, complete sequence.
ACCESSION   EU334820
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G.
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants
  JOURNAL   Plant Methods 4, 3 (2008)
  PUBMED    18211686
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2007) Cell Signalling Laboratory, Stazione 
            Zoologica Anton Dohrn, Villa Comunale, Naples 80121, Italy
REFERENCE   3  (bases 1 to 3030)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Cloning vector pE5n"
                     /mol_type="other DNA"
                     /db_xref="taxon:490925"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    481..1200
                     /label=ECFP tag
                     /note="ECFP tag"
     CDS             481..1197
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1201..1232
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    complement(1259..1358)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1481..2290
                     /codon_start=1
                     /product="Kan(R)"
                     /label=Kan
                     /protein_id="ABY65307.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1673..2346
                     /label=pUC
                     /note="pUC"
     rep_origin      2380..2968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"