LOCUS       Exported               10975 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Integrative expression vector pDYN6902, complete sequence.
ACCESSION   KT265816
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Thibessard A, Bertrand C, Hiblot J, Piotrowski E, Leblond P.
  TITLE     Construction of pDYN6902, a new Streptomyces integrative expression 
            vector designed for cloning sequences interfering with Escherichia 
            coli viability
  JOURNAL   Plasmid (2015) In press
  PUBMED    26476355
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   Thibessard A, Bertrand C, Hiblot J, Piotrowski E, Leblond P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2015) Faculte des Sciences et Technologies, 
            Universite de Lorraine, Boulevard des Aiguillettes Bp 239, 
            Vandoeuvre F-54506, France
REFERENCE   3  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Integrative expression vector pDYN6902"
                     /mol_type="other DNA"
                     /db_xref="taxon:1739551"
     terminator      149..197
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      190..339
                     /regulatory_class="terminator"
                     /function="transcriptional terminator"
                     /note="terminator fd"
     misc_feature    336..407
                     /label=MCS: multiple cloning site
                     /note="MCS: multiple cloning site"
     misc_feature    345..401
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     regulatory      complement(408..515)
                     /regulatory_class="promoter"
                     /function="thiostrepton inducible promoter"
                     /note="pTipA promoter"
     regulatory      538..612
                     /regulatory_class="terminator"
                     /function="transcriptional terminator"
                     /note="terminator t0"
     terminator      572..606
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     complement(628..644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(737..1210)
                     /function="involved in the partition system"
                     /label=SopC binding site
                     /bound_moiety="SopC"
                     /note="sopC is the target site of SopAB complex"
     misc_feature    737..1210
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(1283..2254)
                     /codon_start=1
                     /gene="sopB"
                     /product="SopB"
                     /function="involved in the partition system"
                     /label=sopB
                     /protein_id="ALJ93682.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(1283..2254)
                     /gene="sopB"
                     /label=sopB
     CDS             complement(2254..3429)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             complement(2254..3420)
                     /codon_start=1
                     /gene="sopA"
                     /product="SopA"
                     /function="involved in the partition system"
                     /label=sopA
                     /protein_id="ALJ93683.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDV
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(2254..3420)
                     /gene="sopA"
                     /label=sopA
     misc_feature    3755..4005
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(4008..4763)
                     /codon_start=1
                     /gene="repE"
                     /product="RepE"
                     /function="mediates assembly of a replication complex at 
                     Ori2"
                     /label=repE
                     /note="repA"
                     /protein_id="ALJ93684.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(4008..4763)
                     /gene="repE"
                     /label=repE
     rep_origin      4854..5073
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      complement(5092..5158)
                     /direction=LEFT
                     /note="ori2 (oriS) from the F (fertility) factor of E. 
                     coli"
     CDS             5457..6260
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="apramycin resistance protein"
                     /function="apramycin resistance"
                     /label=aac(3)IV
                     /protein_id="ALJ93685.1"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     gene            5457..6260
                     /gene="aac(3)IV"
                     /label=aac(3)IV
     CDS             5457..6257
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             6729..7538
                     /codon_start=1
                     /gene="tsr"
                     /product="thiostrepton resistance protein"
                     /function="thiostrepton resistance"
                     /label=tsr
                     /protein_id="ALJ93686.1"
                     /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA
                     AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR
                     PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA
                     SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG
                     GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR"
     gene            6729..7538
                     /gene="tsr"
                     /label=tsr
     CDS             complement(7728..8099)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            8132..8243
                     /note="oriT"
     oriT            8132..8241
                     /note="incP origin of transfer"
     CDS             complement(8479..8490)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    8561..8660
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     misc_feature    8591..8629
                     /label=phiC31 attP site
                     /note="phiC31 attP site"
     CDS             8646..10487
                     /codon_start=1
                     /gene="int"
                     /product="integrase"
                     /function="phiC31 integrase"
                     /label=int
                     /protein_id="ALJ93687.1"
                     /translation="MTQGVVTGVDTYAGAYDRQSRERENSSAASPATQRSANEDKAADL
                     QREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSR
                     LKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILD
                     TKNLQRELGGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPD
                     VIRWWWREIKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWD
                     PATVMRILRDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEP
                     AEWYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRK
                     VVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEA
                     PEKSGERANLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEER
                     LAELEAAEAPKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTT
                     GRGQGTPIEKRASITWAKPPTDDDEDDAQDGTEDVAA"
     gene            8646..10487
                     /gene="int"
                     /label=int
     CDS             8670..10487
                     /codon_start=1
                     /product="integrase from phage phi-C31"
                     /label=phage phi-C31 integrase
                     /translation="MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDG
                     GRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIP
                     IVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQREL
                     GGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWRE
                     IKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRIL
                     RDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQA
                     WLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPG
                     QHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERA
                     NLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAE
                     APKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPI
                     EKRASITWAKPPTDDDEDDAQDGTEDVAA"
     primer_bind     10930..10946
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"