LOCUS       Exported                4436 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Phage display vector pdvec0 DNA, complete sequence.
ACCESSION   LC410040
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Makino Y, Ishida S, Itoh N.
  TITLE     Phage display vector pdvec0
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Makino Y, Itoh N.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2018) Contact:Yoshihide Makino Toyama Prefectural 
            Univerisity, Department of Biotechnology, Faculty of Engineering; 
            5180 Kurokawa, Imizu-city, Toyama 939-0398, Japan URL 
            :http://www.pu-toyama.ac.jp/
REFERENCE   3  (bases 1 to 4436)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Phage display vector pdvec0"
                     /mol_type="other DNA"
                     /db_xref="taxon:2267868"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     gene            217..1557
                     /gene="III"
                     /label=III
     misc_feature    217..270
                     /gene="III"
                     /note="gene III signal peptide of Mq3KO7. This region 
                     introduces the express of a protein structural gene 
                     inserted in the downstream of this region."
     misc_feature    271..336
                     /gene="III"
                     /note="A protein structural gene can be inserted in this 
                     region. The inserted gene is expressed as a fusion peptide 
                     of (geneIII signal peptide of M13KO7)-(polypeptide from 
                     inserted sequence)-(geneIII mature peptide M13KO7)."
     misc_feature    337..1557
                     /gene="III"
                     /label=geneIII mature peptide of M13KO7
                     /note="geneIII mature peptide of M13KO7"
     primer_bind     complement(1565..1581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1794..2249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2531..2635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2636..3496
                     /codon_start=1
                     /gene="bla"
                     /product="beta lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="BBF25456.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            2636..3496
                     /gene="bla"
                     /label=bla
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"