LOCUS       Exported                7475 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDV-CYFP, complete sequence.
ACCESSION   EF028668
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 7475)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Cloning vector pDV-CYFP"
                     /lab_host="Dictyostelium discoideum"
                     /mol_type="other DNA"
                     /note="Cloning vector pDV-CYFP is used to fuse target 
                     proteins at the C-terminus to enhanced yellow fluorescent 
                     protein (EYFP)"
                     /db_xref="taxon:409541"
     misc_feature    15..44
                     /label=multipe cloning site
                     /note="multipe cloning site"
     misc_feature    45..758
                     /note="3 end of enhanced green fluorescent protein (EGFP)"
     CDS             45..758
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     regulatory      785..1765
                     /regulatory_class="terminator"
                     /note="2H3 terminator"
     CDS             2498..3328
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /protein_id="ABK40477.1"
                     /translation="MDGEDVQAGSFRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSD
                     AAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWL
                     LLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAG
                     LVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCG
                     RLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2534..3328
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        4218..4322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4323..5183
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /protein_id="ABK40476.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      7167..7475
                     /regulatory_class="promoter"
                     /note="actin15 promoter"