LOCUS       Exported                6402 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pDsRed-N-GW, complete sequence.
ACCESSION   LT726797
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6402)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Mammalian expression vector pDsRed-N-GW"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 8365."
                     /db_xref="taxon:1944965"
     regulatory      complement(109..205)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      109..205
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(259..714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 ori"
     regulatory      715..810
                     /regulatory_class="promoter"
                     /note="ampicillin resistance promoter"
     promoter        741..845
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    811..845
                     /label=5 UTR of amp, incl. RBS
                     /note="5 UTR of amp, incl. RBS"
     promoter        847..1204
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      866..1147
                     /regulatory_class="promoter"
                     /note="SV40 early promoter"
     rep_origin      1055..1190
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      1104..1179
                     /label=SV40 ori
                     /note="SV40 ori"
     CDS             1239..2033
                     /codon_start=1
                     /note="unnamed protein product; Tn5 neomycin resistance"
                     /protein_id="SJL86510.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      2224..2293
                     /regulatory_class="terminator"
                     /note="HSV-TK polyA"
     polyA_signal    2265..2312
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2641..3229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3011..3337
                     /label=pMB1 ori
                     /note="pMB1 ori"
     regulatory      3344..3911
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     enhancer        3404..3707
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3708..3911
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3950..3959
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3956..4630
                     /codon_start=1
                     /note="unnamed protein product; mDsRed"
                     /protein_id="SJL86511.1"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     regulatory      complement(4694..4818)
                     /regulatory_class="attenuator"
                     /note="attR1"
     protein_bind    4694..4818
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      4826..4878
                     /regulatory_class="promoter"
                     /note="lacUV5 promoter"
     promoter        4843..4873
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4927..5586
                     /codon_start=1
                     /note="unnamed protein product; chloramphenicol resistance"
                     /protein_id="SJL86512.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5928..6233
                     /codon_start=1
                     /note="unnamed protein product; ccdB"
                     /protein_id="SJL86513.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      6274..6398
                     /regulatory_class="attenuator"
                     /note="attR2"
     protein_bind    complement(6274..6398)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"