LOCUS       Exported                3543 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG264, complete sequence.
ACCESSION   MH492384
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 3543)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Cloning vector pDSG264"
                     /mol_type="other DNA"
                     /db_xref="taxon:2268478"
     regulatory      2..73
                     /regulatory_class="terminator"
                     /note="his operon terminator"
     terminator      2..59
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     regulatory      10..42
                     /regulatory_class="terminator"
                     /note="terminator stem loop"
     primer_bind     complement(132..154)
                     /label=VR primer site(1)
                     /note="VR primer site(1)"
     misc_feature    complement(137..154)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(217..1032)
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /protein_id="AXC07676.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(1364..2188)
                     /label=p15A ORI
                     /note="p15A ORI"
     rep_origin      complement(1627..2171)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    2589..2609
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      2661..2704
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      2678..2697
                     /regulatory_class="terminator"
                     /note="terminator stem loop"
     misc_feature    2707..2728
                     /label=biobrick prefix
                     /note="biobrick prefix"
     misc_feature    2712..2819
                     /label=insert from pXJ147_pLambda-mRuby2_pSC101.xdna
                     /note="insert from pXJ147_pLambda-mRuby2_pSC101.xdna"
     misc_feature    2729..2777
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    2786..2797
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             2786..2797
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    2804..3523
                     /label=superfolder GFP
                     /note="superfolder GFP"
     CDS             2804..3517
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    3524..1
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    3524..3543
                     /label=biobrick suffix
                     /note="biobrick suffix"