LOCUS       Exported                3560 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG262, complete sequence.
ACCESSION   MH492383
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 3560)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Cloning vector pDSG262"
                     /mol_type="other DNA"
                     /db_xref="taxon:2268476"
     misc_feature    complement(1..3541)
                     /label=biobrick suffix
                     /note="biobrick suffix"
     regulatory      2..75
                     /regulatory_class="terminator"
                     /note="E.coli his operon terminator"
     regulatory      10..44
                     /regulatory_class="terminator"
                     /note="terminator stem loop"
     primer_bind     complement(134..156)
                     /label=VR primer site
                     /note="VR primer site"
     misc_feature    complement(139..156)
                     /label=VR primer site
                     /note="VR primer site"
     CDS             complement(219..1034)
                     /codon_start=1
                     /product="KanR"
                     /label=KanR
                     /protein_id="AXC07675.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    complement(1366..2190)
                     /label=p15A ORI
                     /note="p15A ORI"
     rep_origin      complement(1629..2173)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     misc_feature    2591..2611
                     /label=VF2 primer site
                     /note="VF2 primer site"
     terminator      2663..2706
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      2680..2699
                     /regulatory_class="terminator"
                     /note="terminator stem loop"
     misc_feature    2709..2730
                     /label=biobrick prefix
                     /note="biobrick prefix"
     misc_feature    2731..2779
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    2788..2799
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             2788..2799
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    2806..3540
                     /label=mRuby2
                     /note="mRuby2"
     CDS             2806..3516
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             3517..3534
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3541..1
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"