LOCUS       Exported                4149 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDSG261, complete sequence.
ACCESSION   MH492387
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Glass DS, Riedel-Kruse IH.
  TITLE     A synthetic bacterial cell-cell adhesion toolbox for programming 
            multicellular morphologies and patterns
  JOURNAL   Cell (2018) In press
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Glass DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2018) Bioengineering, Stanford University, 318 
            Campus Drive, Clark Center E350, Stanford, CA 94305, USA
REFERENCE   3  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Cloning vector pDSG261"
                     /mol_type="other DNA"
                     /db_xref="taxon:2268475"
     misc_feature    1..49
                     /label=pLambda (BBa_R0051)
                     /note="pLambda (BBa_R0051)"
     misc_feature    58..69
                     /label=RBS (BBa_B0034)
                     /note="RBS (BBa_B0034)"
     RBS             58..69
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    76..810
                     /label=mRuby2
                     /note="mRuby2"
     CDS             76..786
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             787..804
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    811..831
                     /label=biobrick suffix
                     /note="biobrick suffix"
     regulatory      832..905
                     /regulatory_class="terminator"
     CDS             complement(1194..2144)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /protein_id="AXC07680.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     misc_feature    complement(2148..2160)
                     /label=RBS
                     /note="RBS"
     regulatory      complement(2165..2184)
                     /regulatory_class="promoter"
                     /note="repA promoter"
     rep_origin      2192..2414
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      2906..3000
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3030..3890)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="AXC07681.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3891..3995)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3891..3969)
                     /regulatory_class="promoter"
                     /note="promoter/RBS for AmpR"
     terminator      4082..4125
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4128..4149
                     /label=biobricks prefix
                     /note="biobricks prefix"