LOCUS       Exported                3851 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDRIVE, complete sequence.
ACCESSION   DQ996013
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Rega S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2006) Qiagen, 27220 Turnberry Lane, Suite 200, 
            Valencia, CA 91355, USA
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Cloning vector pDRIVE"
                     /mol_type="other DNA"
                     /db_xref="taxon:404219"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     209..224
                     /label=M13 reverse
                     /note="M13 reverse"
     CDS             216..596
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSNTTHYRESSVPRMLQTRYVSDPEFVISEFVDKLLEPR
                     LALDHTCGGPSSRPLYSIVSPKWPHNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRN
                     SEEARTDRPSQQLRSLNGEWKL"
     misc_feature    216..593
                     /label=lacZ alpha-peptide
                     /note="lacZ alpha-peptide"
     regulatory      239..258
                     /regulatory_class="promoter"
                     /note="T7 RNA polymerase"
     primer_bind     239..258
                     /label=T7 promoter
                     /note="T7 promoter"
     promoter        239..257
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    256
                     /label=T7 transcription start site
                     /note="T7 transcription start site"
     misc_feature    266..393
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      complement(398..417)
                     /regulatory_class="promoter"
                     /note="SP6 RNA polymerase"
     promoter        complement(399..417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(400..418)
                     /label=SP6 promoter
                     /note="SP6 promoter"
     misc_feature    complement(400)
                     /label=SP6 transcription start site
                     /note="SP6 transcription start site"
     primer_bind     complement(431..447)
                     /label=M13 forward (-20)
                     /note="M13 forward (-20)"
     primer_bind     complement(451..467)
                     /label=M13 forward (-40)
                     /note="M13 forward (-40)"
     rep_origin      complement(588..1043)
                     /direction=LEFT
                     /label=phage f1
                     /note="phage f1"
     promoter        1070..1174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1175..2035
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ABI82063.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             2181..2996
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /label=kanamycin resistance protein
                     /protein_id="ABI82064.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3668
                     /label=pUC
                     /note="pUC"