LOCUS       Exported               13276 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDR3-Lcspt-tTA, complete sequence.
ACCESSION   KX278430
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13276)
  AUTHORS   Yan Y, Scott MJ.
  TITLE     Transgenic early-larval sexing systems for genetic control of the 
            Australian sheep blow fly Lucilia cuprina
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13276)
  AUTHORS   Yan Y, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2016) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 13276)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13276
                     /organism="Cloning vector pDR3-Lcspt-tTA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1940699"
     mobile_element  20..798
                     /mobile_element_type="transposon:pBAC 3"
     primer_bind     complement(1497..1513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1987..2091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2092..2952
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3123..3711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3999..4020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4035..4065
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4073..4089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4097..4113
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  4291..4973
                     /mobile_element_type="transposon:pBAC 5"
     protein_bind    5106..5205
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     3UTR           complement(5271..6688)
                     /label=Lctub pA
                     /note="Lctub pA"
     CDS             complement(6695..7390)
                     /codon_start=1
                     /product="ZsGreen"
                     /label=ZsGreen
                     /protein_id="AQA28227.1"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIADYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     regulatory      complement(7391..9604)
                     /regulatory_class="promoter"
                     /note="Lchsp83"
     regulatory      9605..11400
                     /regulatory_class="promoter"
                     /note="Lsspt"
     CDS             11403..12419
                     /codon_start=1
                     /product="tTAv"
                     /label=tTAv
                     /protein_id="AQA28226.1"
                     /translation="MVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSGPAYSRARTKNNYG
                     STIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAM
                     AHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             12183..12416
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     3UTR           12427..13272
                     /label=SV40 pA
                     /note="SV40 pA"
     intron          12504..12569
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12699..12719
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13144..13265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"