LOCUS       Exported               12343 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDR2[Lsbnk-tTAo], complete sequence.
ACCESSION   KT749916
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12343)
  AUTHORS   Yan Y, Scott MJ.
  TITLE     A transgenic embryonic sexing system for the Australian sheep blow 
            fly Lucilia cuprina
  JOURNAL   Sci Rep 5, 16090 (2015)
  PUBMED    26537204
REFERENCE   2  (bases 1 to 12343)
  AUTHORS   Yan Y, Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 12343)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12343
                     /organism="Cloning vector pDR2[Lsbnk-tTAo]"
                     /mol_type="other DNA"
                     /db_xref="taxon:1755133"
     mobile_element  24..802
                     /mobile_element_type="transposon:3 pBAC"
     primer_bind     complement(1501..1517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1991..2095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2096..2956
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3127..3715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4003..4024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4039..4069
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4077..4093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4101..4117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  4295..4977
                     /mobile_element_type="transposon:5 pBAC"
     misc_recomb     5015..5272
                     /label=phiC31 attP
                     /note="phiC31 attP"
     protein_bind    5110..5209
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     regulatory      complement(5275..6692)
                     /regulatory_class="polyA_signal_sequence"
                     /note="Lctub pA"
     CDS             complement(6699..7394)
                     /codon_start=1
                     /gene="ZsGreen"
                     /product="ZsGreen"
                     /label=ZsGreen
                     /note="fluorescent protein"
                     /protein_id="ALO23609.1"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIADYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     gene            complement(6699..7394)
                     /gene="ZsGreen"
                     /label=ZsGreen
     regulatory      complement(7395..9604)
                     /regulatory_class="promoter"
                     /gene="Lchsp83"
     gene            complement(7395..9604)
                     /gene="Lchsp83"
                     /label=Lchsp83
     regulatory      9612..10467
                     /regulatory_class="promoter"
                     /gene="Lsbnk"
     gene            9612..10467
                     /gene="Lsbnk"
                     /label=Lsbnk
     CDS             10474..11490
                     /codon_start=1
                     /gene="tTAo"
                     /product="tTAo"
                     /label=tTAo
                     /protein_id="ALO23610.1"
                     /translation="MVSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSGPAYSRARTKNNYG
                     STIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAM
                     AHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     gene            10474..11490
                     /gene="tTAo"
                     /label=tTAo
     regulatory      11498..12343
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 pA"
     intron          11575..11640
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11770..11790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12215..12336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"