LOCUS       Exported                7790 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pDP28, complete sequence.
ACCESSION   KJ395591
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Manso AS, Iannelli F, Pozzi G, Furi L, Oggioni MR.
  TITLE     The nucleotide sequence of the Streptococcus pneumoniae Escherichia 
            coli shuttle vector pDP28
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Manso AS, Iannelli F, Pozzi G, Furi L, Oggioni MR.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2014) Department of Genetics, University of 
            Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK
REFERENCE   3  (bases 1 to 7790)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Shuttle vector pDP28"
                     /mol_type="other DNA"
                     /db_xref="taxon:1485959"
     misc_feature    1..2392
                     /note="HindIII-SalI fragment of plasmid pSMB1 in INSDC 
                     accession AF047385"
     rep_origin      184..201
                     /note="double strand replication origin of pneumococcal 
                     plasmids pDP1 and pSMB1"
     CDS             403..1365
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /note="REP pDP1 pSMB1"
                     /protein_id="AHX11846.1"
                     /translation="MEKWENQDKILLDKNKRGKDRNWRGRKLLSLKLADIFKELGYRET
                     LIERVETCGDTLRFIRREDGSLRLYQAYFCKNKLCPMCNWRRSMKYSYQTSQIVDEAIK
                     EQPKGRFLFLTLTVKNVPGKELNATISQLTQSFDRLFRRAKVKKNLIGFLRSVEVTHNQ
                     EEETYHPHIHVLMMVKSSYFSGAGDNYVSQEEWGRMWEQSLKVDYVPMVDIRSVKEIGK
                     GLKGAILETAKYPIKPIKLDVENKQVVGDLYNGLYRKRQLGYGGLFKEIRKRLQLSNVE
                     NGDLVYTSDDNDEMSKGTKIVAIWNATKQNYFVKNKGWN"
     gene            403..1365
                     /gene="rep"
                     /label=rep
     rep_origin      2352..2392
                     /note="single strand replication origin of pneumococcal 
                     plasmids pDP1 and pSMB1"
     misc_feature    2393..6010
                     /note="SalI-ClaI fragment of plasmid pACYC184 in INSDC 
                     accession X06403"
     CDS             complement(4047..4706)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AHX11847.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(4047..4706)
                     /gene="cat"
                     /label=cat
     promoter        complement(4707..4809)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      complement(5335..5880)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      5336..5880
                     /label=pACYC184/p15A origin of replication
                     /note="pACYC184/p15A origin of replication"
     misc_feature    6011..7790
                     /note="ClaI-HindIII fragment of plasmid pAMbeta1 in INSDC 
                     accession GU128949"
     CDS             complement(6139..6876)
                     /codon_start=1
                     /gene="ermB"
                     /product="rRNA adenine N-6-methyltransferase"
                     /label=ermB
                     /note="erythromycin resistance"
                     /protein_id="AHX11848.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(6139..6876)
                     /gene="ermB"
                     /label=ermB