LOCUS       Exported                6776 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDonrCbx, complete sequence.
ACCESSION   EU360889
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold 
            SE.
  TITLE     DelsGate, a robust and rapid gene deletion construction method
  JOURNAL   Fungal Genet. Biol. 45 (4), 379-388 (2008)
  PUBMED    18248826
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold 
            SE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2007) Plant Pathology, University of Georgia, 
            Miller Plant Sciences Bldg., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 6776)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Cloning vector pDonrCbx"
                     /mol_type="other DNA"
                     /db_xref="taxon:491855"
     terminator      73..100
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      192..278
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     misc_feature    300..324
                     /label=DonrF primer
                     /note="DonrF primer"
     misc_feature    332..563
                     /label=attP1
                     /note="attP1"
     protein_bind    332..563
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     CDS             complement(959..1264)
                     /codon_start=1
                     /gene="ccdB"
                     /product="DNA gyrase inhibitor"
                     /label=ccdB
                     /note="CcdB"
                     /protein_id="ABY65243.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(959..1264)
                     /gene="ccdB"
                     /label=ccdB
     CDS             complement(1606..2265)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /note="CAT"
                     /protein_id="ABY65244.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(1606..2265)
                     /gene="cat"
                     /label=cat
     promoter        complement(2266..2368)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    complement(2513..2744)
                     /label=attP2
                     /note="attP2"
     protein_bind    complement(2513..2744)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     misc_feature    complement(2769..2792)
                     /label=DonrR primer
                     /note="DonrR primer"
     CDS             2868..3677
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /function="kanamycin resistance"
                     /label=kanamycin resistance protein
                     /protein_id="ABY65245.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3794..4467
                     /label=pUC
                     /note="pUC"
     rep_origin      3820..4408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5589..6476
                     /codon_start=1
                     /gene="cbxR"
                     /product="CbxR"
                     /function="carboxin resistance"
                     /label=cbxR
                     /protein_id="ABY65246.1"
                     /translation="MSLFNVSNGLRTALRPSVASSSRVAAFSTTAAARLATPTSDNVGS
                     SGKPQHLKQFKIYRWNPDKPSEKPRLQSYTLDLNQTGPMVLDALIKIKNEIDPTLTFRR
                     SCREGICGSCAMNIDGVNTLACLCRIDKQNDTKIYPLPHMYIVKDLVPDLTQFYKQYRS
                     IEPFLKSNNTPSEGEHLQSPEERRRLDGLYECILCACCSTSCPSYWWNQDEYLGPAVLM
                     QAYRWMADSRDDFGEERRQKLENTFSLYRCLTIMNCSRTCPKNLNPGKAIAQIKKDMAV
                     GAPKASERPIMASS"
     gene            5589..6476
                     /gene="cbxR"
                     /label=cbxR