LOCUS       Exported                5882 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDONR-A-Hyg, complete sequence.
ACCESSION   EU360963
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold 
            SE.
  TITLE     DelsGate, a robust and rapid gene deletion construction method
  JOURNAL   Fungal Genet. Biol. 45 (4), 379-388 (2008)
  PUBMED    18248826
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold 
            SE.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2007) Plant Pathology, University of Georgia, 
            Miller Plant Sciences Bldg., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Cloning vector pDONR-A-Hyg"
                     /mol_type="other DNA"
                     /db_xref="taxon:491854"
     misc_feature    303..327
                     /label=DonrF primer
                     /note="DonrF primer"
     regulatory      complement(306..5835)
                     /regulatory_class="promoter"
                     /note="TrpC"
     misc_feature    335..566
                     /label=attP1
                     /note="attP1"
     protein_bind    335..566
                     /gene="mutant version of attP"
                     /label=attP1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     CDS             complement(962..1267)
                     /codon_start=1
                     /gene="ccdB"
                     /product="DNA gyrase inhibitor"
                     /label=ccdB
                     /note="CcdB"
                     /protein_id="ABY65247.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(962..1267)
                     /gene="ccdB"
                     /label=ccdB
     CDS             complement(1609..2268)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /function="chloramphenicol resistance"
                     /label=cat
                     /note="CAT"
                     /protein_id="ABY65248.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(1609..2268)
                     /gene="cat"
                     /label=cat
     promoter        complement(2269..2371)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     misc_feature    complement(2516..2747)
                     /label=attP2
                     /note="attP2"
     protein_bind    complement(2516..2747)
                     /gene="mutant version of attP"
                     /label=attP2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)201 version)"
     misc_feature    complement(2772..2795)
                     /label=DonrR primer
                     /note="DonrR primer"
     CDS             2871..3680
                     /codon_start=1
                     /product="kanamycin resistance protein"
                     /function="kanamycin resistance"
                     /label=kanamycin resistance protein
                     /protein_id="ABY65249.1"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3797..4470
                     /label=pUC
                     /note="pUC"
     rep_origin      3823..4411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      4546..4573
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4665..4751
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4805..5830)
                     /codon_start=1
                     /gene="hygR"
                     /product="hygromycin B phosphotransferase"
                     /function="hygromycin B resistance"
                     /label=hygR
                     /protein_id="ABY65250.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            complement(4805..5830)
                     /gene="hygR"
                     /label=hygR