LOCUS       Exported                8596 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pDOJHR, complete sequence.
ACCESSION   DQ123820
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Lee JH, Osullivan DJ.
  TITLE     Sequence Analysis of Two Cryptic Plasmids from Bifidobacterium 
            longum DJO10A and Construction of a Shuttle Cloning Vector
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 527-535 (2006)
  PUBMED    16391088
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Lee J-H., OSullivan DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2005) Food Science and Nutrition, University of 
            Minnesota, 1500 Gortner Ave, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Shuttle vector pDOJHR"
                     /mol_type="other DNA"
                     /note="Bifidobacterium longum/Escherichia coli shuttle 
                     cloning vector"
                     /db_xref="taxon:338057"
     CDS             complement(1870..2328)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="chloramphenicol resistance"
                     /protein_id="AAZ32757.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPEKCFFSFYYSMDFIYWV"
     gene            complement(1870..2328)
                     /gene="cat"
                     /label=cat
     CDS             2791..2802
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(4082..4186)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(4427..4750)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /protein_id="AAZ32758.1"
                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(4427..4750)
                     /gene="lacZ"
                     /label=lacZ
     primer_bind     4660..4676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4678..4734)
                     /gene="lacZ"
                     /label=multiple cloning site from pUC18
                     /note="multiple cloning site from pUC18"
     misc_feature    4680..4736
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4746..4762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4770..4786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4794..4824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4839..4860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        complement(5313..5341)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(5353..6199)
                     /direction=LEFT
                     /label=p15A origin of replication
                     /note="p15A origin of replication"
     rep_origin      5453..5998
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6200..7678)
                     /codon_start=1
                     /gene="mob"
                     /product="mobilization protein"
                     /label=mob
                     /protein_id="AAZ32759.1"
                     /translation="MAIYSLNISSASSAVAALSYITSMRVRDDARGETYSGFGRRERVA
                     HVATLLPEGAPSGYADPERLFNAAQAAERGTGVAAKKIMVALPRELDEGQRVLAVERFI
                     RENLTAGGYAATYAIHLDREGRNPHAHILVANRRIDPRTGEWARLKQKTTFALDGNGQR
                     IPVIDPKTGVQKVDKRNRKQWKRVTVSENPLGTKAMLLSMRESWADVCNGLLPEGVRID
                     HRSLEEQGIDRVPTIHEGYASREMEKRGQPSDRMAINREIGASNRDLAEGDAAVDDLRA
                     HQSELRRLLRDMADRAVQAFDRMLESIGHGVDPSDAVEAMRDRDGINLMIPRNAKDSDR
                     PVWHAWLYDRHEWAAIPSHVLREGMRTVGRVRAAIGTALHRWRDAQAARRERIARSTPA
                     QAPRLADVARQAREDAGQPAHQPRPVRKPEQQPEQQRERPVSLAAAAKEASKQLKREQQ
                     QEPEPEPALEWNPWDPADPMNLGMGGSQGYGLGL"
     gene            complement(6200..7678)
                     /gene="mob"
                     /label=mob
     CDS             8011..8493
                     /codon_start=1
                     /gene="orfIV"
                     /product="hypothetical protein"
                     /label=orfIV
                     /protein_id="AAZ32760.1"
                     /translation="MIEPIGGNEMPKSFAQQIEDDENKIKRIREHQRMVRAKQAKQERN
                     ARTKRLVETGAIVEKAHGGAYDDEGRQTFSDGLNGIISVYDPSRGGNVDMRVIDVIDRR
                     IPRLPRSETTTGTAAAASRTVQATAPQPAHAQPQSFTPNPQRIEHRTGAQQPDRWA"
     gene            8011..8493
                     /gene="orfIV"
                     /label=orfIV
     CDS             8561..876
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AAZ32756.1"
                     /translation="MSEICRIIVAMSTELREQWEQLYLPLRPLCTNDFIEGVYRQPRAK
                     ALEGYRYIEANPKTVSDLLVVDIDDANARAMALWEHEGMLPNVIVENPRNGHAHAVWAL
                     AAPFSRTEYARRKPLALAAAVTEGLRRSCDGDKGYSGLMTKNPLHEAWNSELVTDHLYG
                     LDELREALEESGDMPPASWKRTKRRNVVGLGRNCHLFETARTWAYREVRHHWGDSEGLR
                     LAISAEAHEMNAELFPEPLAWSEVEQIAKSIHKWIVTQSRMWRDGPTVYEATFITVQSA
                     RGKKSGKARRETFELFASEEMT"
     gene            8561..876
                     /gene="rep"
                     /label=rep