LOCUS       Exported                6820 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDMK3, complete sequence.
ACCESSION   FJ824848
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Kadzhaev K, Zingmark C, Golovliov I, Bolanowski M, Shen H, Conlan W,
            Sjostedt A.
  TITLE     Identification of genes contributing to the virulence of Francisella
            tularensis SCHU S4 in a mouse intradermal infection model
  JOURNAL   PLoS ONE 4 (5), E5463 (2009)
  PUBMED    19424499
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Kadzhaev K, Golovliov I, Sjostedt A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2009) Clinical Bacteriology, Umea University, NUS,
            Umea 90185, Sweden
REFERENCE   3  (bases 1 to 6820)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Cloning vector pDMK3"
                     /mol_type="other DNA"
                     /db_xref="taxon:632922"
     protein_bind    120..136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     rep_origin      171..565
                     /label=R6K replication origin
                     /note="R6K replication origin"
     rep_origin      171..559
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     misc_feature    584..2313
                     /label=RP4 mobilization region
                     /note="RP4 mobilization region"
     CDS             complement(585..1142)
                     /codon_start=1
                     /gene="traI"
                     /product="TraI"
                     /label=traI
                     /protein_id="ACO50702.1"
                     /translation="MIAKHVPMRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANT
                     LPAVMAEVMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQR
                     VSAVHHDTDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQ
                     RVSENRANDMERHAGVESLVGWI"
     gene            complement(585..1142)
                     /gene="traI"
                     /label=traI
     CDS             complement(1139..1180)
                     /codon_start=1
                     /gene="traX"
                     /product="TraX"
                     /label=traX
                     /protein_id="ACO50703.1"
                     /translation="MTFLAAKTAGGCA"
     gene            complement(1139..1180)
                     /gene="traX"
                     /label=traX
     CDS             complement(1177..1548)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label=traJ
                     /protein_id="ACO50704.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     gene            complement(1177..1548)
                     /gene="traJ"
                     /label=traJ
     oriT            1581..1690
                     /note="incP origin of transfer"
     CDS             1916..2312
                     /codon_start=1
                     /gene="traK"
                     /product="TraK"
                     /label=traK
                     /protein_id="ACO50705.1"
                     /translation="MPKSYTDELAEWVESRAAKKRRRDEAAVAFLAVRADVEAALASGY
                     ALVTIWEHMRETGKVKFSYETFRSHARRHIKAKPADVPAPQAKAAEPAPAPKTPEPRRP
                     KQGGKAEKPAPAAAPTGFTFNPTPDKKD"
     gene            1916..2312
                     /gene="traK"
                     /label=traK
     promoter        3127..3572
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             3573..4994
                     /codon_start=1
                     /gene="sacB"
                     /product="SacB"
                     /label=sacB
                     /protein_id="ACO50706.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     gene            3573..4994
                     /gene="sacB"
                     /label=sacB
     regulatory      5711..6018
                     /regulatory_class="promoter"
                     /note="groES promoter"
     CDS             6026..6820
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /label=npt
                     /protein_id="ACO50707.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            6026..6820
                     /gene="npt"
                     /label=npt