LOCUS       Exported                4311 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pDIVA, complete sequence.
ACCESSION   KX665539
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Construction of infectious full-length cDNA clones of Beet 
            soil-borne mosaic virus and Beet necrotic yellow vein virus A-type 
            by isothermal in vitro recombination analysis of systemic movement, 
            vector transmissions and transreplication properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Phytomedicine, Leibniz Universitaet Hannover
            - Institute of Horticultural Production Systems, Herrenhaeuser 
            Strasse 2, Hannover 30419, Germany
REFERENCE   3  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Main Workbench version 6.8.1 Sequencing
            Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Binary vector pDIVA"
                     /mol_type="other DNA"
                     /db_xref="taxon:1962271"
     rep_origin      1..630
                     /label=Replication origin
                     /note="Replication origin"
     CDS             839..1633
                     /codon_start=1
                     /product="nptIII"
                     /label=nptIII
                     /note="Aminoglycoside resistance"
                     /protein_id="ARA90383.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1932..3080
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /note="Plasmid replication initiator protein"
                     /protein_id="ARA90384.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3130..3299
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    3235..3259
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    3330..3754
                     /label=35S promoter
                     /note="35S promoter"
     promoter        3709..3754
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    3755..3839
                     /label=HDVagrz
                     /note="HDVagrz"
     misc_feature    3840..3874
                     /label=35 bp spacer
                     /note="35 bp spacer"
     misc_feature    3875..4079
                     /label=pA35S
                     /note="pA35S"
     primer_bind     complement(4109..4125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4153..4309
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    4206..4230
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      4310..630
                     /label=oriV
                     /note="incP origin of replication"