LOCUS       Exported                4475 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDGO144, complete sequence.
ACCESSION   KX259111
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd 
            LR.
  TITLE     Development of a plasmid-based expression system in Clostridium 
            thermocellum and its use to screen heterologous expression of 
            bifunctional alcohol dehydrogenases (adhEs)
  JOURNAL   Metab Eng Commun 3, 120-129 (2016)
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd 
            LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Cloning vector pDGO144"
                     /mol_type="other DNA"
                     /db_xref="taxon:1930526"
     CDS             1..1005
                     /codon_start=1
                     /gene="repB"
                     /product="RepB"
                     /label=repB
                     /note="replication protein"
                     /protein_id="APQ46202.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            1..1005
                     /gene="repB"
                     /label=repB
     misc_feature    1011..1051
                     /label=multiple cloning site
                     /note="multiple cloning site"
     gene            1088..1830
                     /gene="cat"
                     /label=cat
     regulatory      1088..1179
                     /regulatory_class="promoter"
                     /gene="cat"
     CDS             1180..1830
                     /codon_start=1
                     /gene="cat"
                     /product="Cat"
                     /label=cat
                     /note="chloramphenicol transacetylase"
                     /protein_id="APQ46203.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(1908..2768)
                     /codon_start=1
                     /gene="amp"
                     /product="AmpR"
                     /label=amp
                     /note="ampicillin resistance protein"
                     /protein_id="APQ46204.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1908..2768)
                     /gene="amp"
                     /label=amp
     promoter        complement(2769..2860)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3460..4005
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."