LOCUS       Exported                5412 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDGO126ins_p2638-1705, complete sequence.
ACCESSION   KX272603
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in 
            Thermoanaerobacterium saccharolyticum and its role in ethanol 
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
  PUBMED    27694237
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Cloning vector pDGO126ins_p2638-1705"
                     /mol_type="other DNA"
                     /db_xref="taxon:1906601"
     CDS             complement(65..925)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="AOU74528.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(926..1017)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1590..2417
     rep_origin      1617..2162
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             2633..3637
                     /codon_start=1
                     /product="RepB"
                     /label=RepB
                     /protein_id="AOU74530.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     regulatory      3679..3887
                     /regulatory_class="promoter"
                     /note="P2638"
     CDS             3888..4619
                     /codon_start=1
                     /product="putative ferredoxin:NAD+ oxidoreductase"
                     /EC_number="1.18.1.2"
                     /label=putative ferredoxin:NAD+ oxidoreductase
                     /note="Tsac_1705"
                     /protein_id="AOU74527.1"
                     /translation="MRYVVRENREISNGIYRLRLEFDGIPTPGQFIMINCGGNTLLKRP
                     FSICDHDADEITIVYRIKGKGTYNLSKVKALDTLEVTGPHGHGFDMFDKFNNILIVGGG
                     IGIPPLLFLSKKLKSKNLKIALGFKSDVFLVDEFKKYGDVVIATEDGNTGIKGYVTDAM
                     KDEIKDIDMIYGCGPMQMLKSVKDMAEKYDVPCQISIEEHMGCGIGACMVCACKTKTDS
                     GFQYKKVCKDGPVFWSREVVF"
     CDS             4749..5399
                     /codon_start=1
                     /product="CAT"
                     /label=CAT
                     /protein_id="AOU74529.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"