LOCUS       Exported                9045 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  CipA deletion vector pDGO-03, complete sequence.
ACCESSION   JX489218
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Waller BH, Olson DG, Currie DH, Guss AM, Lynd LR.
  TITLE     Exchange of type II dockerin-containing subunits of the Clostridium 
            thermocellum cellulosome as revealed by SNAP-tags
  JOURNAL   FEMS Microbiol. Lett. 338 (1), 46-53 (2013)
  PUBMED    23082914
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2012) Thayer School of Engineering, Dartmouth 
            College, Thayer School of Engineering, Dartmouth College, Hanover, 
            NH 03755, USA
REFERENCE   3  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9045
                     /organism="CipA deletion vector pDGO-03"
                     /mol_type="other DNA"
                     /label=Clostridium thermocellum strain DS11
                     /note="Clostridium thermocellum strain DS11"
                     /db_xref="taxon:1238568"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /note="Escherichia coli origin of replication from plasmid 
                     pUC19"
     CDS             complement(903..1706)
                     /codon_start=1
                     /gene="ura3"
                     /product="URA3"
                     /label=ura3
                     /note="from yeast; results in ura+, 5-FOA sensitive when 
                     present"
                     /protein_id="AFU51880.1"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     gene            complement(903..1706)
                     /gene="ura3"
                     /label=ura3
     promoter        complement(1707..1922)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      1945..2458
                     /label=CEN6/ARSH4 yeast origin of replication
                     /note="CEN6/ARSH4 yeast origin of replication"
     misc_feature    1955..2458
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     misc_feature    complement(2503..3041)
                     /label=3 flank
                     /note="3 flank"
     misc_feature    complement(3042..3583)
                     /label=5 flank
                     /note="5 flank"
     regulatory      complement(3589..3720)
                     /regulatory_class="terminator"
                     /note="hpt"
     CDS             complement(3721..4275)
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label=hpt
                     /protein_id="AFU51881.1"
                     /translation="MINQIKEILVTREELKNNAKELGKRISSDYEGKELVLIGVLKGGV
                     VFFADLIREITIPIDVDFISVSSYGNSTKSSGVVRIIKDIDIDITNKHVLIVEDLVDTG
                     LTLHYLKSMFEARGPKDVKICTALDKPSRRKVDLEIDYKGITIPDKFVVGYGLDYAEKY
                     RNLPDVCVLDSSVYTDKEDMD"
     gene            complement(3721..4275)
                     /gene="hpt"
                     /label=hpt
     CDS             complement(4291..4941)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AFU51882.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            complement(4291..4941)
                     /gene="cat"
                     /label=cat
     regulatory      complement(4942..5469)
                     /regulatory_class="promoter"
                     /note="gapDH promoter region"
     misc_feature    complement(5473..6040)
                     /label=internal flank
                     /note="internal flank"
     CDS             complement(6044..6622)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label=tdk
                     /note="from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AFU51883.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6044..6622)
                     /gene="tdk"
                     /label=tdk
     regulatory      complement(6623..7243)
                     /regulatory_class="promoter"
                     /note="CBP; cellulose-binding protein promoter"
     CDS             complement(7350..8354)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein"
                     /label=repB
                     /protein_id="AFU51879.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     gene            complement(7350..8354)
                     /gene="repB"
                     /label=repB