LOCUS       Exported               10407 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pDG3661, complete sequence.
ACCESSION   AY618310
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     rRNA promoter strength and regulation in Bacillus subtilis: an 
            alternative strategy for the modulation of ribosome synthesis in 
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Bacteriology, University of Wisconsin, 
            Madison, 420 Henry Mall, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Integration vector pDG3661"
                     /lab_host="Bacillus subtilis"
                     /mol_type="other DNA"
                     /label=designed for promoter studies
                     /note="designed for promoter studies"
                     /db_xref="taxon:279697"
     gene            115..635
                     /gene="amyE"
                     /label=amyE
                     /note="amyE front; partial alpha-amylase; similar to 
                     amyE-front in GenBank Accession Number U46196"
     regulatory      751..837
                     /regulatory_class="terminator"
                     /note="derived from Bacillus subtilis rrnB; terminates 
                     transcription from amyE front"
     regulatory      complement(844..932)
                     /regulatory_class="terminator"
                     /note="derived from Bacillus subtilis rrnO; terminates lacZ
                     transcription"
     CDS             complement(947..4006)
                     /codon_start=1
                     /gene="spoVG-lacZ"
                     /product="beta-galactosidase"
                     /label=spoVG-lacZ
                     /note="similar to AAB40294 encoded by GenBank Accession 
                     Number U46196"
                     /protein_id="AAT48878.1"
                     /translation="MEVTDVRLRVDRENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQ
                     QLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPIT
                     VNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRL
                     PSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFH
                     VATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGY
                     ADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLL
                     NGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCD
                     RYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNES
                     GHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIK
                     KWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDE
                     NGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVT
                     SEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTV
                     RVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQ
                     FNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHY
                     QAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASD
                     TPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSE
                     NGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGI
                     GGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(947..4006)
                     /gene="spoVG-lacZ"
                     /label=spoVG-lacZ
     CDS             4391..5041
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetylase"
                     /label=cat
                     /note="chloramphenicol resistance; similar to AAB40295 
                     encoded by GenBank Accession Number U46196"
                     /protein_id="AAT48879.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            4391..5041
                     /gene="cat"
                     /label=cat
     gene            5412..6440
                     /gene="amyE"
                     /label=amyE
                     /note="amyE back; partial alpha-amylase; similar to 
                     amyE-back in GenBank Accession Number U46196"
     CDS             7549..8331
                     /codon_start=1
                     /gene="spc"
                     /product="spectinomycin adenyltransferase"
                     /label=spc
                     /note="spectinomycin resistance; similar to AAB40297 
                     encoded by GenBank Accession Number U46196"
                     /protein_id="AAT48880.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRIN
                     SDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYPPK
                     REFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLTDIR
                     RAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEHVTLL
                     DIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            7549..8331
                     /gene="spc"
                     /label=spc
     rep_origin      complement(8578..9166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9337..10197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance; similar to AAB40298 encoded 
                     by GenBank Accession Number U46196"
                     /protein_id="AAT48881.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(9337..10197)
                     /gene="bla"
                     /label=bla
     promoter        complement(10198..10302)
                     /gene="bla"
                     /label=AmpR promoter