LOCUS       Exported                5768 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDESTR DNA, complete sequence.
ACCESSION   AB218275
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2005) Teruo Sone, Graduate School of Agriculture, 
            Hokkaido University, Laboratory of Applied Microbiology; Kita-9 
            Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 
            (E-mail:sonet@chem.agr.hokudai.ac.jp, Tel:81-11-706-2502, 
            Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Cloning vector pDESTR"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:331619"
     rep_origin      26..481
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        559..663
                     /gene="bla"
                     /label=AmpR promoter
     CDS             664..1524
                     /codon_start=1
                     /gene="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /protein_id="BAE53670.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            664..1524
                     /gene="AmpR"
                     /label=AmpR
     rep_origin      1695..2283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2519..4023
                     /label=Hph casette from pCB1004
                     /note="Hph casette from pCB1004"
     regulatory      2565..2922
                     /regulatory_class="promoter"
                     /gene="TrpC"
     gene            2565..2922
                     /gene="TrpC"
                     /label=TrpC
     CDS             2923..3948
                     /codon_start=1
                     /gene="HygR"
                     /product="HygromycinB phosphotransferase"
                     /label=HygR
                     /protein_id="BAE53671.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     gene            2923..3948
                     /gene="HygR"
                     /label=HygR
     misc_feature    4056..5768
                     /label=GATEWAY CcdB - CmR casette for LR recombination
                     /note="GATEWAY CcdB - CmR casette for LR recombination"
     protein_bind    4060..4184
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4209..4239
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4293..4952
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5294..5599
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5640..5764)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"