LOCUS       Exported               11987 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDC8, complete sequence.
ACCESSION   EU047752
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Xu X.
  TITLE     A pDU1-based plasmid carrying promoterless chloramphenicol acetyl 
            transferase gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Xu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2007) Institute of Hydrobiology, Chinese Academy 
            of Sciences, #7 Donghu South Rd, Wuchang, Wuhan, Hubei 430072, China
REFERENCE   3  (bases 1 to 11987)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Cloning vector pDC8"
                     /mol_type="other DNA"
                     /db_xref="taxon:459738"
     misc_feature    1..6361
                     /label=pDU1 sequence for replication in Nostoc/Anabaena
                     /note="pDU1 sequence for replication in Nostoc/Anabaena"
     CDS             7100..7894
                     /codon_start=1
                     /gene="npt"
                     /product="neomycin phosphotransferase"
                     /label=npt
                     /note="neomycin and kanamycin resistance"
                     /protein_id="ABU42127.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            7100..7894
                     /gene="npt"
                     /label=npt
     misc_feature    8447..8587
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8773..9361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9507..10166
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /note="chloramphenicol resistance"
                     /protein_id="ABU42128.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            9507..10166
                     /gene="cat"
                     /label=cat
     intron          10333..10398
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10529..10549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"