LOCUS       Exported                4496 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  Cloning vector pDC123, complete sequence.
ACCESSION   AF167172
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Rothschild CB, Ross RP, Claiborne A.
  TITLE     Molecular analysis of the gene encoding alkaline phosphatase in 
            Streptococcus faecalis 10C1
  JOURNAL   (in) Dunny,G., Cleary,P. and McKay,L. (Eds.); GENETICS AND MOLECULAR
            BIOLOGY OF STREPTOCOCCI,LACTOCOCCI AND ENTEROCOCCI: 179-183; 
            American Society for Microbiology, Washington, DC, USA (1991)
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Chaffin DO, Rubens CE.
  TITLE     Blue/white screening of recombinant plasmids in Gram-positive 
            bacteria by interruption of alkaline phosphatase gene (phoZ) 
            expression
  JOURNAL   Gene 219 (1-2), 91-99 (1998)
  PUBMED    9757005
REFERENCE   3  (bases 1 to 4496)
  AUTHORS   Lee MH, Nittayajarn A, Ross RP, Rothschild CB, Parsonage D, 
            Claiborne A, Rubens CE.
  TITLE     Characterization of Enterococcus faecalis alkaline phosphatase and 
            use in identifying Streptococcus agalactiae secreted proteins
  JOURNAL   J. Bacteriol. 181 (18), 5790-5799 (1999)
  PUBMED    10482522
REFERENCE   4  (bases 1 to 4496)
  AUTHORS   Chaffin DO, Rubens CE.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-1999) Division of Infectious Disease, Immunology 
            and Rheumatology, Childrens Hospital and Regional Medical Center, 
            4800 Sand Point Way N.E., Seattle, WA 98105, USA
REFERENCE   5  (bases 1 to 4496)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Cloning vector pDC123"
                     /mol_type="other DNA"
                     /db_xref="taxon:102901"
     gene            complement(46..1559)
                     /gene="phoZMCS"
                     /label=phoZMCS
                     /experiment="experimental evidence, no additional details 
                     recorded"
     CDS             complement(46..1542)
                     /codon_start=1
                     /gene="phoZMCS"
                     /product="alkaline phosphatase"
                     /label=phoZMCS
                     /note="PhoZMCS; from Entrococcus faecalis; contains the 
                     in-frame insertion of a MCS to facilitate introduction of 
                     foreign DNA"
                     /citation="[1]"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAD53269.1"
                     /translation="MKKRALLGVTLLTFTTLAGCTNLSEQKSGEKQTEVACGTRARGAL
                     DIDAYEFSCRILVSLACAEAKATESEKAPVKNVIFMIGDGMGNPYTTGYRYFKANHSDK
                     RVPQTAFDTYLVGQQATYPEDEEENVTDSASAATAMAAGVKTYNNAIALDNDKSKTETV
                     LERAKKVGKSTGLVATSEITHATPAAYGAHNVSRKNMAEIADDYFDDQIDGQHKVDVLL
                     GGGSELFARKDRDLVKEFSQAGYGHVTDKKSLNENQDDKILGLFAPGGLPKMIDRTEEV
                     PSLADMTEAALQRLDKNEKGFFLMVEGSQIDWAGHSNDIVGAMSEMQDFEAAFEKAIDF
                     AKKDGETLVVTTADHSTGGLSLGKGDQYNWLTEPLHAAKRTPDFMAEEIIKNGNVEKTV
                     TEYIDFQLSEAELKAVKTAAESKDVEKIAQALRKIFDERSNTGWTTGGHTGEDVNVYAY
                     GPQAEAFSGQIDNTDQAKIIFGLVDGTGQKAEIKDKGIGK"
     misc_feature    1357..1437
                     /label=multiple cloning site
                     /note="multiple cloning site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      complement(1552..1558)
                     /regulatory_class="ribosome_binding_site"
                     /gene="phoZMCS"
     gene            complement(1571..2313)
                     /gene="cat"
                     /label=cat
                     /experiment="experimental evidence, no additional details 
                     recorded"
     CDS             complement(1571..2221)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl transferase"
                     /label=cat
                     /note="Cat; from Staphylococcus aureus plasmid pC194"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAD53270.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     regulatory      complement(2285..2290)
                     /regulatory_class="minus_10_signal"
                     /gene="cat"
     regulatory      complement(2308..2313)
                     /regulatory_class="minus_35_signal"
                     /gene="cat"
     regulatory      complement(2494..2499)
                     /regulatory_class="minus_10_signal"
                     /note="from tet promoter"
     regulatory      complement(2516..2521)
                     /regulatory_class="minus_35_signal"
                     /note="from tet promoter"
     gene            complement(2553..3251)
                     /gene="repB"
                     /label=repB
     regulatory      complement(2553..2573)
                     /regulatory_class="terminator"
                     /gene="repB"
     CDS             complement(2619..3251)
                     /codon_start=1
                     /gene="repB"
                     /product="topoisomerase"
                     /function="topoisomerase"
                     /label=repB
                     /note="RepB; from Streptococcus agalactiae pMV158 (pLS1); 
                     site specific DNA strand transferase"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAD53271.1"
                     /translation="MAKEKARYFTFLLYPESIPSDWELKLETLGVPMAISPLHDKDKSS
                     IKGQKYKKAHYHVLYIAKNPVTADSVRKKIKLLLGEKSLAMVQVVLNVENMYLYLTHES
                     KDAIAKKKHVYDKADIKLINNFDIDRYVTLDVEEKTELFNVVVSLIRAYTLQNIFDLYD
                     FIDENGETYGLTINLVNEVIAGKTGFMKLLFDGAYQRSKRGTKNEER"
     gene            3232..3318
                     /gene="rnaII"
                     /label=rnaII
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      3233..3238
                     /regulatory_class="minus_35_signal"
                     /gene="rnaII"
     regulatory      3256..3261
                     /regulatory_class="minus_10_signal"
                     /gene="rnaII"
     misc_RNA        3268..3318
                     /gene="rnaII"
                     /product="antisense regulatory RNA"
                     /note="counter transcribed regulatory RNA; ctII"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     gene            complement(3312..3511)
                     /gene="copG"
                     /label=copG
                     /note="formerly known as repA"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     CDS             complement(3312..3449)
                     /codon_start=1
                     /gene="copG"
                     /product="transcriptional repressor"
                     /function="regulation of plasmid copy number"
                     /label=copG
                     /note="CopG; formerly known as RepA; from Streptococcus 
                     agalactiae pMV158 (pLS1)"
                     /experiment="experimental evidence, no additional details 
                     recorded"
                     /protein_id="AAD53272.1"
                     /translation="MKKRLTITLSESVLENLEKMAREMGLSKSAMISVALENYKKGQEK
                     "
     regulatory      complement(3456..3461)
                     /regulatory_class="ribosome_binding_site"
                     /gene="copG"
     regulatory      complement(3478..3483)
                     /regulatory_class="minus_10_signal"
                     /gene="copG"
     misc_feature    3493..3505
                     /note="copG operator; DNA binding site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     regulatory      complement(3501..3506)
                     /regulatory_class="minus_35_signal"
                     /gene="copG"
     protein_bind    3539..3573
                     /label=RepB binding site
                     /bound_moiety="RepB"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     rep_origin      complement(3614..3694)
                     /direction=LEFT
                     /label=double stranded origin (dso) of replication
                     /note="double stranded origin (dso) of replication"
     misc_feature    3655..3662
                     /note="nic; RepB DNA nick site"
     rep_origin      4294..4409
                     /label=single stranded origin (sso) of replication
                     /note="single stranded origin (sso) of replication"