LOCUS       Exported                6619 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDBR, complete sequence.
ACCESSION   FR822201
VERSION     .
KEYWORDS    complete replicon.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Calderon-Gomez E, Lampropoulou V, Shen P, Neves P, Roch T, Stervbo 
            U, Rutz S, Kuehl AA, Heppner FL, Loddenkemper C, Charneau P, 
            Fillatreau S.
  TITLE     Reprogrammed quiescent B cells provide an effective cellular therapy
            against chronic experimental encephalomyelitis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Fillatreau S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2011) Fillatreau S., Immune Regulation, Deutsche 
            Rheuma-ForschungsZentrum, a Leibniz Institute, Chariteplatz 1, 
            D-10117, GERMANY
REFERENCE   3  (bases 1 to 6619)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Cloning vector pDBR"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:985816"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      239..815
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     repeat_region   835..1015
                     /label=HIV 5 LTR
                     /note="HIV 5 LTR"
     LTR             835..1015
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1126..1170
                     /label=HIV PSI pack
                     /note="HIV PSI pack"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2448..2463
                     /label=HIV cPPT/FLAP
                     /note="HIV cPPT/FLAP"
     misc_feature    2657..2690
                     /label=LoxP
                     /note="LoxP"
     protein_bind    2657..2690
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2755..3307
                     /regulatory_class="promoter"
                     /note="CMV promoter"
     enhancer        2806..3109
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3110..3313
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    3345..3396
                     /note="multiple cloning site (BamH1, Xba1, Pme1, Ecor1, 
                     Xho1)"
     misc_feature    3419..3452
                     /label=LoxP
                     /note="LoxP"
     protein_bind    3419..3452
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    3508..4096
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    3510..4097
                     /label=WPRE sequence
                     /note="WPRE sequence"
     CDS             complement(3979..3990)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4099..4115)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4625..4805
                     /label=3 SIN LTR
                     /note="3 SIN LTR"
     LTR             4625..4805
                     /label=5 LTR (truncated)
                     /note="truncated 5 long terminal repeat (LTR) from HIV-1"
     misc_feature    4852..5471
                     /label=pBR322 origin replication
                     /note="pBR322 origin replication"
     rep_origin      complement(4867..5455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5626..6486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="CBZ41582.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5626..6486)
                     /gene="bla"
                     /label=bla
     promoter        complement(6487..6591)
                     /gene="bla"
                     /label=AmpR promoter