LOCUS       Exported               11660 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCYPAC7, complete sequence.
ACCESSION   DQ092493
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11660)
  AUTHORS   Ferrier DEK., Dewar K, Amemiya CT.
  TITLE     The chordate ParaHox gene cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11660)
  AUTHORS   Ferrier DEK., Dewar K, Amemiya CT.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2005) Molecular Genetics, Benaroya Research 
            Institute at Virginia, 1201 Ninth Avenue, Seattle, WA 98101, USA
REFERENCE   3  (bases 1 to 11660)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11660
                     /organism="Cloning vector pCYPAC7"
                     /mol_type="other DNA"
                     /db_xref="taxon:334821"
     misc_feature    1..6
                     /label=BamHI cloning site
                     /note="BamHI cloning site"
     promoter        complement(7..25)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             125..1543
                     /codon_start=1
                     /product="SacB"
                     /label=SacB
                     /note="levan sucrase gene for selection of recombinants on 
                     sucrose"
                     /protein_id="AAY98425.1"
                     /translation="MNIKKIVKQATVLTFTTALLAGGATQAFAKENNQKAYKETYGVSH
                     ITRHDMLQIPKQQQNEKYQVPQFDQSTIKNIESAKGLDVWDSWPLQNADGTVAEYNGYH
                     VVFALAGSPKDADDTSIYMFYQKVGDNSIDSWKNAGRVFKDSDKFDANDPILKDQTQEW
                     SGSATFTSDGKIRLFYTDYSGKHYGKQSLTTAQVNVSKSDDTLKINGVEDHKTIFDGDG
                     KTYQNVQQFIDEGNYTSGDNHTLRDPHYVEDKGHKYLVFEANTGTENGYQGEESLFNKA
                     YYGGGTNFFRKESQKLQQSAKKRDAELANGALGIIELNNDYTLKKVMKPLITSNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGINSNDIYMLGYVSNSLTGPYKPLNKTGLVLQ
                     MGLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFFEDKKATFAPSFLMNIKGNKTSV
                     VKNSILEQGQLTVN"
     promoter        complement(1965..1993)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     protein_bind    complement(2022..2055)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      2056..10164
                     /label=P1 bacteriophage origin of replication
                     /note="P1 bacteriophage origin of replication"
     CDS             complement(10463..11278)
                     /codon_start=1
                     /product="aminoglycoside 3-phosphotransferase"
                     /label=aminoglycoside 3-phosphotransferase
                     /protein_id="AAY98424.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     regulatory      11589..11629
                     /regulatory_class="promoter"
                     /note="Escherichia coli promoter to drive SacB gene in 
                     absence of insert; for SacB selection"
     promoter        11638..11656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"