LOCUS       Exported                6248 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral vector pCXneo DNA, complete sequence.
ACCESSION   AB041928
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Akagi T, Shishido T, Murata K, Hanafusa H.
  TITLE     v-Crk activates the phosphoinositide 3-kinase/AKT pathway in 
            transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (13), 7290-7295 (2000)
  PUBMED    10852971
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute, 
            The First Department; 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850, 
            Fax:81-6-6871-6686)
REFERENCE   3  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Retroviral vector pCXneo"
                     /mol_type="other DNA"
                     /db_xref="taxon:197519"
     regulatory      1..466
                     /regulatory_class="enhancer"
                     /note="CMV enhancer"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label=R-U5 region of MuLV 5LTR
                     /note="R-U5 region of MuLV 5LTR"
     misc_feature    763..1673
                     /note="psi+; MuLV extended packaging signal"
     misc_feature    826..1183
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1248..1664
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1674..2049
                     /label=MuLV pol region containing splice acceptor site
                     /note="MuLV pol region containing splice acceptor site"
     misc_feature    1674..2048
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2052..2136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      2137..2757
                     /regulatory_class="ribosome_binding_site"
                     /note="IRES sequence from ECMV"
     CDS             2751..3545
                     /codon_start=1
                     /gene="neo"
                     /function="neomycin resistance"
                     /label=neo
                     /protein_id="BAA94847.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            2751..3545
                     /gene="neo"
                     /label=neo
     LTR             3605..4198
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     repeat_region   3605..4197
                     /label=3 LTR of MuLV
                     /note="3 LTR of MuLV"
     rep_origin      complement(4435..5023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5187..6047)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="BAA94848.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5187..6047)
                     /gene="bla"
                     /label=bla
     promoter        complement(6048..6152)
                     /gene="bla"
                     /label=AmpR promoter