LOCUS       Exported                6787 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCXGFP-1 DNA, complete sequence.
ACCESSION   AB281497
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Suemizu H.
  TITLE     Expression vector pCXGFP-1 DNA, complete sequence
  JOURNAL   Published Only in Database (2006)
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Suemizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2006) Contact:Hiroshi Suemizu Central Institute 
            for Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   3  (bases 1 to 6787)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Expression vector pCXGFP-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:411961"
     regulatory      7..142
                     /regulatory_class="enhancer"
                     /note="polyoma virus enhancer"
     regulatory      149..281
                     /regulatory_class="promoter"
                     /note="HSV-TK promoter"
     CDS             286..1089
                     /codon_start=1
                     /gene="neo"
                     /product="neomycin resistance protein"
                     /label=neo
                     /protein_id="BAF36705.1"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            286..1089
                     /gene="neo"
                     /label=neo
     misc_feature    1090..1144
                     /label=DNA fragment contains SV40 polyA signal
                     /note="DNA fragment contains SV40 polyA signal"
     polyA_signal    1096..1144
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      1151..1534
                     /regulatory_class="enhancer"
                     /note="CMV IE enhancer"
     enhancer        1154..1533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      1535..1901
                     /regulatory_class="promoter"
                     /note="chicken beta-actin promoter"
     promoter        1536..1811
                     /label=chicken beta-actin promoter
     intron          1812..2820
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          1902..2772
                     /note="chicken beta-actin 1st intron"
     intron          2773..2820
                     /note="rabbit beta-globin 2nd intron"
     exon            2821..2874
                     /note="rabbit beta-globin 3rd exon"
     misc_feature    2875..2880
                     /label=cloning site EcoRV
                     /note="cloning site EcoRV"
     CDS             2881..3006
                     /codon_start=1
                     /gene="MICA"
                     /product="truncated MHC class I chain related gene A 
                     protein"
                     /label=MICA
                     /note="transmembrane domain; truncated MICA protein"
                     /protein_id="BAF36706.1"
                     /translation="QSHWQTFHVSAVAAAAAAIFVIIIFYVRCCKKKTSGDPPVAT"
     gene            2881..3006
                     /gene="MICA"
                     /label=MICA
     regulatory      3001..3010
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3007..3726
                     /codon_start=1
                     /gene="EGFP"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /protein_id="BAF36707.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            3007..3726
                     /gene="EGFP"
                     /label=EGFP
     misc_feature    3728..4277
                     /note="DNA fragment contains polyA signal; rabbit 
                     beta-globin 3-flanking sequence"
     polyA_site      3876..3931
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(4290..4306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4314..4330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4338..4368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4383..4404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4463..4658
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4509..4644
                     /label=SV40 replication origin
                     /note="SV40 replication origin"
     polyA_signal    4664..4798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5004..5647
                     /label=pUC plasmid replication origin
                     /note="pUC plasmid replication origin"
     rep_origin      complement(5037..5625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5796..6656)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="BAF36708.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5796..6656)
                     /gene="amp"
                     /label=amp
     promoter        complement(6657..6761)
                     /gene="bla"
                     /label=AmpR promoter