LOCUS       Exported                5883 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral vector pCXbsr DNA, complete sequence.
ACCESSION   AB041927
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Akagi T, Shishido T, Murata K, Hanafusa H.
  TITLE     v-Crk activates the phosphoinositide 3-kinase/AKT pathway in 
            transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (13), 7290-7295 (2000)
  PUBMED    10852971
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute, 
            The First Department; 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850, 
            Fax:81-6-6871-6686)
REFERENCE   3  (bases 1 to 5883)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Retroviral vector pCXbsr"
                     /mol_type="other DNA"
                     /db_xref="taxon:197518"
     regulatory      1..466
                     /regulatory_class="enhancer"
                     /note="CMV enhancer"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label=R-U5 region of MuLV 5LTR
                     /note="R-U5 region of MuLV 5LTR"
     misc_feature    763..1673
                     /note="psi+; extended viral packaging signal"
     misc_feature    826..1183
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1248..1664
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1674..2049
                     /label=pol region containing splice accetor site
                     /note="pol region containing splice accetor site"
     misc_feature    1674..2048
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2052..2136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      2137..2757
                     /regulatory_class="ribosome_binding_site"
                     /note="IRES sequence from ECMV"
     misc_feature    2185..2756
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     exon            2759..3190
                     /gene="bsr"
     gene            2759..3190
                     /gene="bsr"
                     /label=bsr
     CDS             2759..3187
                     /codon_start=1
                     /gene="bsr"
                     /function="blasticidin S resistance"
                     /label=bsr
                     /protein_id="BAA94845.1"
                     /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI
                     ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM
                     CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN"
     LTR             3240..3833
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     repeat_region   3240..3832
                     /label=3 LTR of MuLV
                     /note="3 LTR of MuLV"
     rep_origin      complement(4070..4658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     exon            complement(4224..5682)
                     /gene="bla"
     gene            complement(4224..5682)
                     /gene="bla"
                     /label=bla
     CDS             complement(4822..5682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="BAA94846.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5683..5787)
                     /gene="bla"
                     /label=AmpR promoter