LOCUS       Exported                7724 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCST5-act1, complete sequence.
ACCESSION   HQ536213
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Cote P, Sulea T, Dignard D, Wu C, Whiteway M.
  TITLE     Evolutionary reshaping of fungal mating pathway scaffold proteins
  JOURNAL   MBio 2 (1), e00230-10 (2011)
  PUBMED    21249169
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Cote P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2010) Health Sector, CNRC-BRI, 6100 Royalmount 
            Av., Montreal, QC H4P2R2, Canada
REFERENCE   3  (bases 1 to 7724)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Cloning vector pCST5-act1"
                     /mol_type="other DNA"
                     /db_xref="taxon:978310"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1124..1936
                     /codon_start=1
                     /product="URA3"
                     /label=URA3
                     /note="selectable marker"
                     /protein_id="ADW40668.1"
                     /translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTKE
                     FLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNTVK
                     KQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSVGSL
                     AYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLGQQYR
                     TVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
     primer_bind     2060..2076
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3068..4219)
                     /codon_start=1
                     /product="Cst5"
                     /label=Cst5
                     /note="similar to Candida albicans STE5"
                     /protein_id="ADW40669.1"
                     /translation="MLQSTPKSWKDFLKSPKKPTSPTTPPGSVTITPNTTPPSVPCVSP
                     RPAKIPSRDPFQTINPGLNLSFANILNKSLANHETCFICGELLSTVFASERILKLNCGD
                     STHSECFKAYFKEDIPHARIHGKTITFAKTCRGLNCSGTRNVVVDVNNWHLVSARKLSL
                     IPKRPAPPHPNSVNINALKTTLQNSDIPTRSPSPDPTVSTTTEVDAYEVETVRNQLIKY
                     LLDLCPKINLSRLVLLGNLRVADELSVCVEPLDYFQTRYVYLFENYMVIWNSVDYPVFV
                     PMQNIQISSRGSSILQVRQKDDGLSTLIQSTSSTVVEKWVVAISDAHLQLPAPDITSTI
                     DTSPSDSDSDIDSDEEVIQQALTKNNWADLMIEIDNALLTSDP"
     regulatory      complement(4427..5450)
                     /regulatory_class="promoter"
                     /note="ACT1"
     promoter        complement(5483..5501)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5522..5538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5546..5562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5570..5600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5615..5636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5924..6512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7231..7609)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5-AACGTT-3 (Sato et al., 1996)"