LOCUS       Exported                5887 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Knock-in vector pCSR1, complete sequence.
ACCESSION   DQ907525
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Bardiya N, Shiu PK.
  TITLE     Cyclosporin A-resistance based gene placement system for Neurospora 
            crassa
  JOURNAL   Fungal Genet. Biol. 44 (5), 307-314 (2007)
  PUBMED    17320431
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Bardiya N, Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2006) Biological Sciences, University of Missouri,
            103 Tucker Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 5887)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Knock-in vector pCSR1"
                     /mol_type="other DNA"
                     /label=gene placement system for Neurospora crassa
                     /note="gene placement system for Neurospora crassa"
                     /db_xref="taxon:400031"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      21..327
                     /label=f(-) ori
                     /note="f(-) ori"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(659..1680)
                     /label=csr-1 3 flank
                     /note="csr-1 3 flank"
     misc_feature    1681..1774
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     1697..1713
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1747..1763)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(1775..3674)
                     /label=csr-1 5 flank
                     /note="csr-1 5 flank"
     promoter        complement(3699..3717)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3738..3754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(3743..3864)
                     /regulatory_class="promoter"
                     /note="derived from lac gene"
     protein_bind    3762..3778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3786..3816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3831..3852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4084..4751
                     /label=pUC origin
                     /note="pUC origin"
     rep_origin      complement(4140..4728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4899..5759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="ABK01324.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4899..5759)
                     /gene="bla"
                     /label=bla
     promoter        complement(5760..5864)
                     /gene="bla"
                     /label=AmpR promoter