LOCUS       Exported               11437 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCsGFPBT, complete sequence.
ACCESSION   DQ370426
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     A Gateway adapted Agrobacterium binary vector series for plant 
            biological studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11437)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   3  (bases 1 to 11437)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Binary vector pCsGFPBT"
                     /mol_type="other DNA"
                     /db_xref="taxon:370585"
     misc_feature    1..2434
                     /label=derived from plasmid pGFP-JS
                     /note="derived from plasmid pGFP-JS"
     regulatory      1..758
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus 35S promoter; duplicated"
     promoter        697..743
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     5UTR           759..894
                     /note="translation leader sequence; 5UTR from tobacco etch
                     virus"
     CDS             895..1623
                     /codon_start=1
                     /product="sGFP"
                     /label=sGFP
                     /note="modified GFP by the Sheen Lab with enhanced 
                     fluorescence"
                     /protein_id="ABD16422.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYKGIL"
     CDS             895..1611
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      1624..1835
                     /regulatory_class="polyA_signal_sequence"
                     /note="cauliflower mosaic virus 35S poly(A) signal"
     primer_bind     complement(1857..1873)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2435..11437
                     /note="derived from plasmid pCAMBIA-1302 of GenBank 
                     Accession Number AF234298"
     terminator      2466..2718
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      2469..2721
                     /regulatory_class="polyA_signal_sequence"
                     /note="nopaline synthase poly(A) signal"
     misc_feature    2740..2764
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     repeat_region   2759..2784
                     /label=right border T-DNA repeat
                     /note="right border T-DNA repeat"
     misc_feature    complement(3825..4825)
                     /label=STA region from pVS1 plasmid
                     /note="STA region from pVS1 plasmid"
     CDS             4064..4693
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5127..6194
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      complement(5418..6418)
                     /direction=LEFT
                     /note="pVS1-REP; replication origin from pVS1"
     rep_origin      6260..6454
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6798..6938
                     /label=bom
                     /note="basis of mobility region from pBR322"
     misc_feature    complement(6828..7088)
                     /label=bom site from pBR322
                     /note="bom site from pBR322"
     rep_origin      complement(7124..7712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      complement(7228..7508)
                     /direction=LEFT
                     /label=pBR322 replication origin
                     /note="pBR322 replication origin"
     CDS             complement(7799..8593)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=aminoglycoside phosphotransferase
                     /note="aadA (kanamycin resistance) amplified from pIG121Hm"
                     /protein_id="ABD16421.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     repeat_region   9018..9043
                     /label=left border repeat from C58 T-DNA
                     /note="left border repeat from C58 T-DNA"
     misc_feature    9018..9042
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      complement(9110..9318)
                     /regulatory_class="polyA_signal_sequence"
                     /note="cauliflower mosaic virus 35S poly(A) signal"
     polyA_signal    9120..9294
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9334..10359)
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /label=hygromycin phosphotransferase
                     /note="hptII (hygromycin resistance)"
                     /protein_id="ABD16423.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      complement(10395..11175)
                     /regulatory_class="promoter"
                     /note="CaMV35S2; CaMV 35S promoter; duplicated"
     promoter        complement(10427..11104)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11295..11316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      11323..11377
                     /regulatory_class="promoter"
                     /note="lacZ promoter"
     promoter        11331..11361
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11369..11385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11393..11409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"