LOCUS       Exported               10365 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCS4-BASK, complete sequence.
ACCESSION   HQ593861
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection 
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
  PUBMED    21366919
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 10365)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Binary vector pCS4-BASK"
                     /mol_type="other DNA"
                     /db_xref="taxon:980181"
     misc_feature    complement(1..25)
                     /label=Agrobacterium T-DNA left border
                     /note="Agrobacterium T-DNA left border"
     regulatory      complement(145..304)
                     /regulatory_class="terminator"
                     /note="CaMV35S polyA terminator"
     CDS             complement(359..1300)
                     /codon_start=1
                     /gene="Sul1"
                     /product="Sul1 dihydropteroate synthase"
                     /label=Sul1
                     /note="sulfonamide resistance protein; confers resistance 
                     to sulfadiazine in plants"
                     /protein_id="ADW81560.1"
                     /translation="MGPFGGLKSMTGFPVKKVNTDITSITSNGGRVKCMVTVFGILNLT
                     EDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVGPAASHPDARPVSPADEIRRIAPLLD
                     ALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQGFPDPALYPDIAEADCRLVVMHSAQ
                     RDGIATRTGHLRPEDALDEIVRFFEARVSALRRSGVAADRLILDPGMGFFLSPAPETSL
                     HVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLPVKDLGPASLAAELHAIGNGADYVRT
                     HAPGDLRSAITISETLAKFRSRDARDRGLDHA"
     gene            complement(359..1300)
                     /gene="Sul1"
                     /label=Sul1
                     /note="SUL1"
     regulatory      complement(1307..2053)
                     /regulatory_class="promoter"
                     /note="double enhance CaMV35S promoter"
     promoter        complement(1307..1651)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     gene            2078..3671
                     /gene="Ubi"
                     /label=Ubi
                     /note="ubiquitin"
     regulatory      2078..2866
                     /regulatory_class="promoter"
                     /gene="Ubi"
                     /note="derived from Solanum bulbocastanum 409S; bul409S"
     5UTR           2867..3443
                     /gene="Ubi"
     intron          2910..3443
                     /gene="Ubi"
                     /note="bul409S Ubiquitin 5 intron"
     misc_feature    3444..3671
                     /gene="Ubi"
                     /note="ubitquitin monomer; designed for an N-terminal 
                     translational fusion with genes of interest for 
                     constitutive overexpression in transgenic plants; derived 
                     from Solanum bulbocastanum 409S ubiquitin monomer"
     terminator      3753..4005
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     regulatory      3756..4005
                     /regulatory_class="terminator"
                     /note="nopaline synthase (nos) polyA terminator"
     misc_feature    complement(4087..4111)
                     /label=Agrobacterium T-DNA right border
                     /note="Agrobacterium T-DNA right border"
     misc_feature    4112..10365
                     /label=pCAMBIA0390 binary vector backbone
                     /note="pCAMBIA0390 binary vector backbone"
     CDS             5412..6041
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6475..7542
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7608..7802
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8146..8286
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8472..9060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9147..9941)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"