LOCUS       Exported                8420 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCS, complete sequence.
ACCESSION   HQ593860
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Novel sulI binary vectors enable an inexpensive foliar selection 
            method in Arabidopsis
  JOURNAL   BMC Res Notes 4 (1), 44 (2011)
  PUBMED    21366919
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Thomson JG, Cook M, Guttman M, Smith J, Thilmony R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2010) Crop Improvement and Utilization Research 
            Unit, USDA-ARS-WRRC, 800 Buchanan Street, Albany, CA 94710-1105, USA
REFERENCE   3  (bases 1 to 8420)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Binary vector pCS"
                     /mol_type="other DNA"
                     /db_xref="taxon:980180"
     misc_feature    complement(1..25)
                     /label=Agrobacterium T-DNA left border
                     /note="Agrobacterium T-DNA left border"
     regulatory      complement(149..308)
                     /regulatory_class="terminator"
                     /note="CaMV35S polyA terminator"
     CDS             complement(363..1304)
                     /codon_start=1
                     /gene="Sul1"
                     /product="RbcS-Sul1 fusion precursor"
                     /label=Sul1
                     /protein_id="ADW81559.1"
                     /translation="MGPFGGLKSMTGFPVKKVNTDITSITSNGGRVKCMVTVFGILNLT
                     EDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVGPAASHPDARPVSPADEIRRIAPLLD
                     ALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQGFPDPALYPDIAEADCRLVVMHSAQ
                     RDGIATRTGHLRPEDALDEIVRFFEARVSALRRSGVAADRLILDPGMGFFLSPAPETSL
                     HVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLPVKDLGPASLAAELHAIGNGADYVRT
                     HAPGDLRSAITISETLAKFRSRDARDRGLDHA"
     gene            complement(363..1304)
                     /gene="Sul1"
                     /label=Sul1
                     /note="SUL1"
     mat_peptide     complement(366..1202)
                     /gene="Sul1"
                     /product="sulfonamide resistance protein"
                     /note="confers resistance to sulfadiazine in plants"
     transit_peptide complement(1203..1304)
                     /gene="Sul1"
                     /note="pea rbcS ribulose-bisphosphate carboxylase small 
                     subunit transit peptide for plastid targeting"
     regulatory      complement(1311..2057)
                     /regulatory_class="promoter"
                     /note="double enhanced CaMV35S promoter"
     promoter        complement(1311..1655)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(2142..2166)
                     /label=Agrobacterium T-DNA right border
                     /note="Agrobacterium T-DNA right border"
     misc_feature    2167..8420
                     /label=pCAMBIA0390 binary vector backbone
                     /note="pCAMBIA0390 binary vector backbone"
     CDS             3467..4096
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4530..5597
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5663..5857
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6201..6341
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6527..7115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7202..7996)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"