LOCUS       Exported                6433 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCRR1, complete sequence.
ACCESSION   AF020539
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Zhao Y, Goriparthi L, Lieberman HB.
  TITLE     A new shuttle vector system for the identification of spontaneous 
            and radiation-induced mutations in the fission yeast 
            Schizosaccharomyces pombe
  JOURNAL   Mutat. Res. 311 (1), 111-123 (1994)
  PUBMED    7526164
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Li J, Zaider M, Zhao Y, Ladik J.
  TITLE     Ab initio electronic structure calculation of a new gene system 
            using the negative factor counting method
  JOURNAL   Radiat. Res. 149 (5), 416-421 (1998)
  PUBMED    9588351
REFERENCE   3  (bases 1 to 6433)
  AUTHORS   Zhao Y, Goriparthi L, Lieberman HB.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-1997) Pediatrics and Microbiology-Immunology, 
            Northwestern University Medical School, 2430 N. Halsted Street, 
            #218, Chicago, IL 60614, USA
REFERENCE   4  (bases 1 to 6433)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Shuttle vector pCRR1"
                     /plasmid="pCRR1"
                     /host="Escherichia coli; Schizosaccharomyces pombe; 
                     Saccharomyces cerevisiae"
                     /mol_type="genomic DNA"
                     /note="1-426, Escherichia coli supF; 427-2461, Escherichia 
                     coli tetracycline resistance gene; 2462-3666, 
                     Schizosaccharomyces pombe ars1; 3667-5882, Saccharomyces 
                     cerevisiae LEU2 gene; 5883-6433, pBR322 sequence plasmid"
                     /db_xref="taxon:65821"
     tRNA            75..254
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     CDS             486..1676
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      2815..3596
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     CDS             complement(4145..5239)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5240..5644)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        complement(6192..6296)
                     /gene="bla"
                     /label=AmpR promoter