LOCUS       Exported                5688 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector pCRE-Luc, complete sequence.
ACCESSION   AF053461
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     pCRE-Luc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   3  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   4  (bases 1 to 5688)
  AUTHORS   Zheng C-F.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1999) Technical Services, Stratagene, 11011 N. 
            Torrey Pines, San Diego, CA 92037, USA
REFERENCE   5  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Jan 26, 1999 this sequence version replaced gi:3808229.
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Reporter vector pCRE-Luc"
                     /host="Escherichia coli K12"
                     /mol_type="genomic DNA"
                     /note="designed for detection of activation of the cyclic 
                     AMP response element (CRE) pathway in eukaryotic cells"
                     /db_xref="taxon:74544"
     promoter        32..136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             137..997
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1934..2074
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2539..2671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2750..2781
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2808..4460
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4591..4656
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5226..5360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"