LOCUS       Exported                4970 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cre-lox Univector acceptor vector pCR704, complete sequence.
ACCESSION   FJ750580
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Jia F, Gampala SS, Mittal A, Luo Q, Rock CD.
  TITLE     Cre-lox univector acceptor vectors for functional screening in 
            protoplasts: analysis of Arabidopsis donor cDNAs encoding ABSCISIC 
            ACID INSENSITIVE1-like protein phosphatases
  JOURNAL   Plant Mol. Biol. 70 (6), 693-708 (2009)
  PUBMED    19499346
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Rock CD, Jia F, Gampala SSL., Mittal A, Luo Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2009) Biological Science, Texas Tech University, 
            2500 Broadway, Lubbock, TX 79409, USA
REFERENCE   3  (bases 1 to 4970)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Cre-lox Univector acceptor vector pCR704"
                     /mol_type="other DNA"
                     /db_xref="taxon:622364"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        411..2402
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     regulatory      414..2420
                     /regulatory_class="promoter"
                     /note="maize ubiquitin (Ubi-1) promoter"
     misc_feature    2421..2480
                     /label=2 x FLAG epitope tag
                     /note="2 x FLAG epitope tag"
     CDS             2427..2450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2454..2477
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2481..2514
                     /label=loxH recombination site
                     /note="loxH recombination site"
     protein_bind    2481..2514
                     /label=loxH
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT). The loxH variant was optimized for 
                     expression constructs but remains compatible with loxP."
     regulatory      2515..2734
                     /regulatory_class="terminator"
                     /note="Cauliflower mosaic virus (CaMV) 35S terminator"
     misc_feature    2735..413
                     /label=pUC18 cloning vector
                     /note="pUC18 cloning vector"
     primer_bind     complement(2749..2765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2773..2789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2797..2827)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2842..2863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3151..3739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3910..4770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4771..4875)
                     /gene="bla"
                     /label=AmpR promoter